UniProt Accession

 NDKB_HUMAN; P22392; A8MWA3; Q1WM23; Q6LCT6 

Genbank Protein ID

 X58965; M36981; L16785; DQ109675; AC005839; BC002476; BC133029; BC133031; U29200 

Genbank Nucleotide ID

 CAB37870.1; AAA36369.1; AAA60228.1; AAZ82097.1; AAH02476.1; AAI33030.1; AAI33032.1; AAA86745.1 

Protein Name

 Nucleoside diphosphate kinase B 

Protein Synonyms/Alias

 NDK B; NDP kinase B; C-myc purine-binding transcription factor PUF; Histidine protein kinase NDKB; nm23-H2 

Gene Name

 NME2 

Gene Synonyms/Alias

 NM23B 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
12	ERTFIAIKPDGVQRG	ubiquitination	[1]
39	GFRLVAMKFLRASEE	ubiquitination	[2]
49	RASEEHLKQHYIDLK	ubiquitination	[2]
56	KQHYIDLKDRPFFPG	ubiquitination	[2, 3]
85	WEGLNVVKTGRVMLG	ubiquitination	[2]
100	ETNPADSKPGTIRGD	ubiquitination	[1]
124	IHGSDSVKSAEKEIS	ubiquitination	[1, 2, 3]
128	DSVKSAEKEISLWFK	acetylation	[4]
128	DSVKSAEKEISLWFK	ubiquitination	[2, 3]
135	KEISLWFKPEELVDY	ubiquitination	[3]

Reference

 [1] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 

Functional Description

 Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity. 

Sequence Annotation

 REGION 2 66 Interaction with AKAP13.
 ACT_SITE 118 118 Pros-phosphohistidine intermediate.
 BINDING 12 12 ATP.
 BINDING 60 60 ATP.
 BINDING 88 88 ATP.
 BINDING 94 94 ATP.
 BINDING 105 105 ATP.
 BINDING 115 115 ATP.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 94 94 Phosphothreonine (By similarity).
 MOD_RES 128 128 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Activator; Alternative splicing; ATP-binding; Cell projection; Complete proteome; Cytoplasm; Direct protein sequencing; DNA-binding; Kinase; Magnesium; Metal-binding; Nucleotide metabolism; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 152 AA 

Protein Sequence

Gene Ontology

 GO:0071944; C:cell periphery; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0030027; C:lamellipodium; IDA:HGNC.
 GO:0005634; C:nucleus; NAS:UniProtKB.
 GO:0001726; C:ruffle; IDA:HGNC.
 GO:0005524; F:ATP binding; NAS:UniProtKB.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004550; F:nucleoside diphosphate kinase activity; IDA:UniProtKB.
 GO:0004673; F:protein histidine kinase activity; IEA:EC.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:UniProtKB.
 GO:0007155; P:cell adhesion; TAS:HGNC.
 GO:0006241; P:CTP biosynthetic process; IEA:InterPro.
 GO:0006183; P:GTP biosynthetic process; IEA:InterPro.
 GO:0007229; P:integrin-mediated signaling pathway; IDA:UniProtKB.
 GO:0043066; P:negative regulation of apoptotic process; IMP:HGNC.
 GO:0015949; P:nucleobase-containing small molecule interconversion; TAS:Reactome.
 GO:0009142; P:nucleoside triphosphate biosynthetic process; IDA:UniProtKB.
 GO:0050679; P:positive regulation of epithelial cell proliferation; IMP:HGNC.
 GO:0045618; P:positive regulation of keratinocyte differentiation; IMP:HGNC.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006228; P:UTP biosynthetic process; IEA:InterPro. 

Interpro

 IPR001564; Nucleoside_diP_kinase.
 IPR023005; Nucleoside_diP_kinase_AS. 

Pfam

 PF00334; NDK 

SMART

 SM00562; NDK 

PROSITE

 PS00469; NDP_KINASES 

PRINTS

 PR01243; NUCDPKINASE.