CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024782
UniProt Accession
Genbank Protein ID
  
Genbank Nucleotide ID
  
Protein Name
 Inactive serine/threonine-protein kinase TEX14 
Protein Synonyms/Alias
 Testis-expressed sequence 14; Testis-expressed sequence 14 protein 
Gene Name
 Tex14 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
930SKWRLAVKEMAEKAVacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 
Functional Description
 Required both for the formation of intercellular bridges during meiosis and for kinetochore-microtubule attachment during mitosis. Intercellular bridges are evolutionarily conserved structures that connect differentiating germ cells and are required for spermatogenesis and male fertility. Acts by promoting the conversion of midbodies into intercellular bridges via its interaction with CEP55: interaction with CEP55 inhibits the interaction between CEP55 and PDCD6IP/ALIX and TSG101, blocking cell abscission and leading to transform midbodies into intercellular bridges. Also plays a role during mitosis: recruited to kinetochores by PLK1 during early mitosis and regulates the maturation of the outer kinetochores and microtubule attachment. Has no protein kinase activity in vitro (By similarity). 
Sequence Annotation
 REPEAT 27 54 ANK 1.
 REPEAT 55 84 ANK 2.
 REPEAT 88 117 ANK 3.
 DOMAIN 199 512 Protein kinase.
 NP_BIND 205 213 ATP (By similarity).
 MOTIF 788 794 GPPX3Y.
 MOTIF 886 894 D-box.
 BINDING 267 267 ATP (By similarity).
 MOD_RES 431 431 Phosphoserine; by PLK1 (By similarity).  
Keyword
 ANK repeat; ATP-binding; Cell cycle; Cell division; Centromere; Chromosome; Complete proteome; Cytoplasm; Kinetochore; Mitosis; Nucleotide-binding; Phosphoprotein; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1443 AA 
Protein Sequence
MSRAVHFPVP CPVLLGTLTD DSLEAQLHEY AKQGNYVKVK KILKKGVCVD AVNTLGQSAL 60
FVAALLGYVK LVDVLVDYGS DPNHRCFDGS TPVHAAAFSG NQWILSKVLT AGGDLRLHDE 120
KGRNPQAWAL AAGKDRSTQM VEFMQRCASH MKAVIQGFSY DLLKKIDSPQ RLIGSPPWFG 180
GLIQGSPNSS PNRQPKPGII SAQNIYSFGF GKFYLTSGMQ LTYPGSLPVI GEKEVIQADD 240
EPTFSFFSGP YMVMTNLVWN RSRVTVKELN LPTHPHCSRL RLADLLIAEQ EHSSNLRHPS 300
LLQLMAVCLS RDLEKIRLVY ERITVGTLFS VLHERRSQFP VLHMEMIVHL LLQIADALIY 360
LHSRGFIHRS LSSYAVHIVS AGEARLTNLE YMMESQDSGA HRDMTRVPLP AQLYNWAAPE 420
VVLQKTATVK SDIYSFSMII QEILTDNIPW NGLDGSLVKE TIALGNYLEA DVRLPEPYYD 480
IVKSGIHAKQ KNRTMNLQDI RYILKNDLKE FIGAQKTQTT ESPRGQRYEP HPDINICLGL 540
TSEYQKDPPD LDIKELKETG SQPHSPTDHS FLTEKTLAPQ TLDSSLSAQE PDNPNVPSAS 600
RLAEEVRSPT PSEASLYSFE INEIYSGCLT LGTDKEEECP GTASSPEGDK ANQVDELPSL 660
EEELDKMERE LHCFCEEDKS FSDVDTDLSF EDGDWQSDSL SSLSLPEPTR EAKGKTSSWS 720
KTDEYVSKCV LNLKISQVMM QQSAEWLRKL EQEVDELEWA QKELDNQCRS LWDASLRFAN 780
TKFLSAVGPP SLTYLPPVMQ LSEPKQPENG GNWLTLARPP GNERDFQEGN FSKKSEKLSA 840
CDWNPFTQAS EESRGYCSEP NNQPPTLCGP GKQNTGEQLP STEEAKESLE RNTNQNIRRM 900
ESVSSEVCDT KSRNNEDAGE ARSKWRLAVK EMAEKAVSGQ LLVPPWNLQS SFESKVENES 960
TPLPRPPIRG PESTDWQYIL EYQREKDELK GNMKFGKMEN SDCDKNKHSS WTGLQRFTSI 1020
IYPFFRNHEQ PEHNEASQAS CDTXCGEKFY STSSPIEDDF ERIQSSFAQP QGYVEENFQI 1080
REIFGKNAEI LTKPQFQAVQ CAEDEQEETL EETPKELKEK NISLTDIQDL SSITYEQDGC 1140
FKETSCKTPK LKHAPTSAST PLSPESISSA ASHYEDCLEN TFHVKRGSTF CWNGQEAMRT 1200
LSTKFTTVRE RAKSLESLLA SSKTLPAKLT DPKRLCMFGG ACSSNISTAF VTSTHSTKRK 1260
SLPGELAEAT SQHHIDELPP PAQELLDEIE QLKQQQVSSM VSLENTAHDL NVTENDKKHL 1320
EEQETNSSKD SSFLSSREIQ DLEDTERAHS SLDEDLERLL QLPEENMTLL DPTKGSTREK 1380
KTKDQDMVEQ KRKKKEGIKP ERRKSDSFLG TLEEDELKPC FWKRLGWSEP SRIIVLDQSD 1440
LSD 1443 
Gene Ontology
 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
 GO:0045171; C:intercellular bridge; ISS:UniProtKB.
 GO:0000776; C:kinetochore; ISS:UniProtKB.
 GO:0030496; C:midbody; ISS:UniProtKB.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004672; F:protein kinase activity; IEA:InterPro.
 GO:0008608; P:attachment of spindle microtubules to kinetochore; ISS:UniProtKB.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0043063; P:intercellular bridge organization; ISS:UniProtKB.
 GO:0007140; P:male meiosis; ISS:UniProtKB.
 GO:0051306; P:mitotic sister chromatid separation; ISS:UniProtKB.
 GO:0007094; P:mitotic spindle assembly checkpoint; ISS:UniProtKB. 
Interpro
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR001245; Ser-Thr/Tyr_kinase_cat_dom. 
Pfam
 PF12796; Ank_2
 PF07714; Pkinase_Tyr 
SMART
 SM00248; ANK 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM 
PRINTS