CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-041975
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Dual-specificity mitogen-activated protein kinase kinase 2 
Protein Synonyms/Alias
 Mitogen-activated protein kinase kinase 2, isoform CRA_b 
Gene Name
 MAP2K2 
Gene Synonyms/Alias
 hCG_23544 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
4****MARKLIHLEIKubiquitination[1, 2]
11KLIHLEIKPAIRNQIubiquitination[1, 2, 3, 4, 5]
99QIMHRDVKPSNILVNubiquitination[1, 4]
112VNSRGEIKLCDFGVSubiquitination[4]
255FVNKCLIKNPAERADubiquitination[1]
264PAERADLKMLTNHTFubiquitination[1]
273LTNHTFIKRSEVEEVubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Kinase; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 303 AA 
Protein Sequence
MARKLIHLEI KPAIRNQIIR ELQVLHECNS PYIVGFYGAF YSDGEISICM EHMDGGSLDQ 60
VLKEAKRIPE EILGKVSIAV LRGLAYLREK HQIMHRDVKP SNILVNSRGE IKLCDFGVSG 120
QLIDSMANSF VGTRSYMAPE RLQGTHYSVQ SDIWSMGLSL VELAVGRYPI PPPDAKELEA 180
IFGRPVVDGE EGEPHSISPR PRPPGRPVSG HGMDSRPAMA IFELLDYIVN EPPPKLPNGV 240
FTPDFQEFVN KCLIKNPAER ADLKMLTNHT FIKRSEVEEV DFAGWLCKTL RLNQPGTPTR 300
TAV 303 
Gene Ontology
 GO:0005938; C:cell cortex; IEA:Compara.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0004708; F:MAP kinase kinase activity; IEA:Compara.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:InterPro.
 GO:0000187; P:activation of MAPK activity; IEA:GOC.
 GO:2000147; P:positive regulation of cell motility; IEA:Compara. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
  
PROSITE
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS