| Tag | Content |
|---|
| CPLM ID | CPLM-007871 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit STT3A |
Protein Synonyms/Alias | Oligosaccharyl transferase subunit STT3A; STT3-A; B5; Integral membrane protein 1 |
Gene Name | Stt3a |
Gene Synonyms/Alias | Itm1 |
Created Date | July 27, 2013 |
Organism | Mus musculus (Mouse) |
NCBI Taxa ID | 10090 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 565 | AMASTEEKAYEIMRE | ubiquitination | [1] | | 613 | TETGRHIKENDYYTP | ubiquitination | [1] |
|
Reference | [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues. Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C. Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [ PMID: 22790023] |
Functional Description | Catalytic subunit of the N-oligosaccharyl transferase (OST) complex which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. N-glycosylation occurs cotranslationally and the complex associates with the Sec61 complex at the channel-forming translocon complex that mediates protein translocation across the endoplasmic reticulum (ER). SST3A seems to be involved in complex substrate specificity. STT3A is present in the majority of OST complexes and mediates cotranslational N-glycosylation of most sites on target proteins, while STT3B-containing complexes are required for efficient cotranslational glycosylation and mediate glycosylation of sites that have been skipped by STT3A (By similarity). |
Sequence Annotation | CARBOHYD 537 537 N-linked (GlcNAc...) (Potential).
CARBOHYD 544 544 N-linked (GlcNAc...) (Potential).
CARBOHYD 548 548 N-linked (GlcNAc...). |
Keyword | Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Glycoprotein; Glycosyltransferase; Membrane; Reference proteome; Transferase; Transmembrane; Transmembrane helix. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 705 AA |
Protein Sequence | MTKLGFLRLS YEKQDTLLKL LILSMAAVLS FSTRLFAVLR FESVIHEFDP YFNYRTTRFL 60
AEEGFYKFHN WFDDRAWYPL GRIIGGTIYP GLMITSAAIY HVLHFFHITI DIRNVCVFLA 120
PLFSSFTTIV TYHLTKELKD AGAGLLAAAM IAVVPGYISR SVAGSYDNEG IAIFCMLLTY 180
YMWIKAVKTG SIYWAAKCAL AYFYMVSSWG GYVFLINLIP LHVLVLMLTG RFSHRIYVAY 240
CTVYCLGTIL SMQISFVGFQ PVLSSEHMAA FGVFGLCQIH AFVDYLRSKL NPQQFEVLFR 300
SVISLVGFVL LTVGALLMLT GKISPWTGRF YSLLDPSYAK NNIPIIASVS EHQPTTWSSY 360
YFDLQLLVFM FPVGLYYCFS NLSDARIFII MYGVTSMYFS AVMVRLMLVL APVMCILSGI 420
GVSQVLSTYM KNLDISRPDK KSKKQQDSTY PIKNEVASGM ILVMAFFLIT YTFHSTWVTS 480
EAYSSPSIVL SARGGDGSRI IFDDFREAYY WLRHNTPEDA KVMSWWDYGY QITAMANRTI 540
LVDNNTWNNT HISRVGQAMA STEEKAYEIM RELDVSYVLV IFGGLTGYSS DDINKFLWMV 600
RIGGSTETGR HIKENDYYTP TGEFRVDREG SPVLLNCLMY KMCYYRFGQV YTEAKRPPGF 660
DRVRNAEIGN KDFELDVLEE AYTTEHWLVR IYKVKDLDNR GLSRT 705 |
Gene Ontology | GO:0016021; C:integral to membrane; IEA:UniProtKB-KW. GO:0008250; C:oligosaccharyltransferase complex; ISS:UniProtKB. GO:0004579; F:dolichyl-diphosphooligosaccharide-protein glycotransferase activity; ISS:UniProtKB. GO:0043686; P:co-translational protein modification; ISS:UniProtKB. GO:0018279; P:protein N-linked glycosylation via asparagine; ISS:UniProtKB. |
Interpro | |
Pfam | |
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PROSITE | |
PRINTS | |