UniProt Accession

 RIFK_HUMAN; Q969G6; Q5JSG9; Q9NUT7 

Genbank Protein ID

 AK002011; AL391868; BC007069 

Genbank Nucleotide ID

 BAA92033.1; CAI40676.1; AAH07069.1 

Protein Name

 Riboflavin kinase 

Protein Synonyms/Alias

 ATP:riboflavin 5'-phosphotransferase; Flavokinase 

Gene Name

 RFK 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
60	VGSGDVHKMVVSIGW	acetylation	[1]

Reference

 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 

Functional Description

 Catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin-mononucleotide (FMN), hence rate-limiting enzyme in the synthesis of FAD. Essential for TNF-induced reactive oxygen species (ROS) production. Through its interaction with both TNFRSF1A and CYBA, physically and functionally couples TNFRSF1A to NADPH oxidase. TNF-activation of RFK may enhance the incorporation of FAD in NADPH oxidase, a critical step for the assembly and activation of NADPH oxidase. 

Sequence Annotation

 ACT_SITE 79 79 Nucleophile (By similarity).
 METAL 27 27 Magnesium.
 BINDING 15 15 ATP; via amide nitrogen.
 BINDING 21 21 ATP; via amide nitrogen.
 BINDING 27 27 ATP; via amide nitrogen.
 BINDING 29 29 ATP.
 BINDING 82 82 ATP; via amide nitrogen and carbonyl
 BINDING 84 84 ATP; via carbonyl oxygen.
 BINDING 91 91 ATP.
 BINDING 104 104 FMN.
 BINDING 107 107 FMN; via amide nitrogen and carbonyl
 BINDING 109 109 FMN; via amide nitrogen.  

Keyword

 3D-structure; ATP-binding; Complete proteome; Cytoplasm; FMN; Kinase; Magnesium; Metal-binding; Nucleotide-binding; Reference proteome; Transferase; Zinc. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 155 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0008531; F:riboflavin kinase activity; NAS:UniProtKB.
 GO:0009398; P:FMN biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0009231; P:riboflavin biosynthetic process; NAS:UniProtKB. 

Interpro

 IPR023468; Riboflavin_kinase.
 IPR015865; Riboflavin_kinase_bac/euk.
 IPR023465; Riboflavin_kinase_domain. 

Pfam

 PF01687; Flavokinase 

SMART

 SM00904; Flavokinase 

PROSITE

  

PRINTS