CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-029127
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Ubiquitin carboxyl-terminal hydrolase 
Protein Synonyms/Alias
  
Gene Name
 USP14 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
49ARQKVMVKGGTLKMEubiquitination[1]
84IRSVPELKDALKRYAubiquitination[2, 3, 4]
88PELKDALKRYAGALRubiquitination[3]
168MMRVLQQKLEAIEDDubiquitination[2, 3, 4, 5, 6, 7, 8]
178AIEDDSVKETDSSSAubiquitination[3, 4, 5, 6, 8]
192ASAATPSKKKSLIDQubiquitination[3, 5, 6, 8]
193SAATPSKKKSLIDQFubiquitination[4]
221SEEEEVTKGKENQLQubiquitination[3, 4, 9]
223EEEVTKGKENQLQLSubiquitination[3, 4, 6]
245KYLFTGLKLRLQEEIacetylation[10]
245KYLFTGLKLRLQEEIubiquitination[2, 3, 5, 6, 8, 9]
254RLQEEITKQSPTLQRubiquitination[2, 3, 4, 5, 6, 8, 9]
267QRNALYIKSSKISRLacetylation[10]
267QRNALYIKSSKISRLubiquitination[3, 4, 5, 8, 9]
270ALYIKSSKISRLPAYubiquitination[4]
288QMVRFFYKEKESVNAubiquitination[3]
290VRFFYKEKESVNAKVubiquitination[3]
296EKESVNAKVLKDVKFubiquitination[2, 3, 4, 5]
299SVNAKVLKDVKFPLMubiquitination[3]
302AKVLKDVKFPLMLDMubiquitination[3]
329VSFRSKFKDLEDKKVubiquitination[3, 4]
334KFKDLEDKKVNQQPNubiquitination[3]
345QQPNTSDKKSSPQKEacetylation[4]
354SSPQKEVKYEPFSFAubiquitination[3, 6]
395GHYVSWVKRKQDEWIubiquitination[2, 3, 4, 5, 6, 8, 9]
397YVSWVKRKQDEWIKFubiquitination[2]
403RKQDEWIKFDDDKVSubiquitination[2, 3, 5, 6, 8]
408WIKFDDDKVSIVTPEubiquitination[1, 4, 5, 8, 9]
Reference
 [1] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [7] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [8] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [9] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [10] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Hydrolase; Protease; Reference proteome; Thiol protease; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 448 AA 
Protein Sequence
MPLYSVTVKW GKEKFEGVEL NTDEPPMVFK AQLFALTGVQ PARQKVMVKG GTLKMELPCG 60
LTNLGNTCYM NATVQCIRSV PELKDALKRY AGALRASGEM ASAQYITAAL RDLFDSMDKT 120
SSSIPPIILL QFLHMAFPQF AEKGEQGQYL QQDANECWIQ MMRVLQQKLE AIEDDSVKET 180
DSSSASAATP SKKKSLIDQF FGVEFETTMK CTESEEEEVT KGKENQLQLS CFINQEVKYL 240
FTGLKLRLQE EITKQSPTLQ RNALYIKSSK ISRLPAYLTI QMVRFFYKEK ESVNAKVLKD 300
VKFPLMLDMY ELCTPELQEK MVSFRSKFKD LEDKKVNQQP NTSDKKSSPQ KEVKYEPFSF 360
ADDIGSNNCG YYDLQAVLTH QGRSSSSGHY VSWVKRKQDE WIKFDDDKVS IVTPEDILRL 420
SGGGDWHIAY VLLYGPRRVE IMEEESEQ 448 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
 GO:0004221; F:ubiquitin thiolesterase activity; IEA:InterPro.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 
Interpro
 IPR018200; Pept_C19ubi-hydrolase_C_CS.
 IPR001394; Peptidase_C19.
 IPR000626; Ubiquitin. 
Pfam
 PF00443; UCH 
SMART
 SM00213; UBQ 
PROSITE
 PS00972; UCH_2_1
 PS00973; UCH_2_2
 PS50235; UCH_2_3 
PRINTS