CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013441
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Centrosomal protein of 55 kDa 
Protein Synonyms/Alias
 Cep55; Up-regulated in colon cancer 6 
Gene Name
 CEP55 
Gene Synonyms/Alias
 C10orf3; URCC6 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
22GSKPSNSKSETTLEKubiquitination[1]
29KSETTLEKLKGEIAHubiquitination[1]
31ETTLEKLKGEIAHLKubiquitination[1, 2, 3]
129SEEKDVLKQQLSAATubiquitination[4]
145RIAELESKTNTLRLSubiquitination[1, 2, 3, 4, 5]
180QLKDALEKNQQWLVYubiquitination[1, 2, 4]
196QQREVYVKGLLAKIFubiquitination[1, 4]
201YVKGLLAKIFELEKKubiquitination[1, 2]
207AKIFELEKKTETAAHubiquitination[1]
208KIFELEKKTETAAHSubiquitination[1]
221HSLPQQTKKPESEGYubiquitination[1]
222SLPQQTKKPESEGYLubiquitination[1]
233EGYLQEEKQKCYNDLubiquitination[1, 2]
235YLQEEKQKCYNDLLAubiquitination[1]
245NDLLASAKKDLEVERubiquitination[1]
246DLLASAKKDLEVERQubiquitination[1]
268ELSEFRRKYEETQKEubiquitination[1]
274RKYEETQKEVHNLNQubiquitination[1]
303DDRHKTEKIQKLREEubiquitination[1]
306HKTEKIQKLREENDIubiquitination[1]
367TLDFENEKLDRQHVQubiquitination[1, 3, 5]
398ITQLESLKQLHEFAIubiquitination[1, 2, 3]
427EKVAASPKSPTAALNubiquitination[1]
442ESLVECPKCNIQYPAubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
 Plays a role in mitotic exit and cytokinesis. Not required for microtubule nucleation. Recruits PDCD6IP and TSG101 to midbody during cytokinesis. 
Sequence Annotation
 REGION 157 236 Interaction with TSG101.
 REGION 160 214 Interaction with PDCD6IP.
 REGION 355 464 Required for localization to the
 MOD_RES 425 425 Phosphoserine; by CDK1 and MAPK1.
 MOD_RES 428 428 Phosphoserine; by CDK1 and MAPK1.
 MOD_RES 430 430 Phosphothreonine.
 MOD_RES 436 436 Phosphoserine; by PLK1.  
Keyword
 3D-structure; Alternative splicing; Cell cycle; Cell division; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Mitosis; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 464 AA 
Protein Sequence
MSSRSTKDLI KSKWGSKPSN SKSETTLEKL KGEIAHLKTS VDEITSGKGK LTDKERHRLL 60
EKIRVLEAEK EKNAYQLTEK DKEIQRLRDQ LKARYSTTTL LEQLEETTRE GERREQVLKA 120
LSEEKDVLKQ QLSAATSRIA ELESKTNTLR LSQTVAPNCF NSSINNIHEM EIQLKDALEK 180
NQQWLVYDQQ REVYVKGLLA KIFELEKKTE TAAHSLPQQT KKPESEGYLQ EEKQKCYNDL 240
LASAKKDLEV ERQTITQLSF ELSEFRRKYE ETQKEVHNLN QLLYSQRRAD VQHLEDDRHK 300
TEKIQKLREE NDIARGKLEE EKKRSEELLS QVQFLYTSLL KQQEEQTRVA LLEQQMQACT 360
LDFENEKLDR QHVQHQLHVI LKELRKARNQ ITQLESLKQL HEFAITEPLV TFQGETENRE 420
KVAASPKSPT AALNESLVEC PKCNIQYPAT EHRDLLVHVE YCSK 464 
Gene Ontology
 GO:0005814; C:centriole; IEA:UniProtKB-SubCell.
 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0032154; C:cleavage furrow; IEA:UniProtKB-SubCell.
 GO:0045171; C:intercellular bridge; IEA:Compara.
 GO:0030496; C:midbody; IDA:MGI.
 GO:0000910; P:cytokinesis; IGI:MGI.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW. 
Interpro
 IPR022008; EABR. 
Pfam
 PF12180; EABR 
SMART
  
PROSITE
  
PRINTS