CPLM-019801

Genbank Nucleotide ID

Calcium and integrin-binding protein 1

Protein Synonyms/Alias

CIB; Calcium- and integrin-binding protein; CIBP; Calmyrin; DNA-PKcs-interacting protein; Kinase-interacting protein; KIP; SNK-interacting protein 2-28; SIP2-28

Homo sapiens (Human)

Position	Peptide	Type	References
10	GSGSRLSKELLAEYQ	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8]
24	QDLTFLTKQEILLAH	ubiquitination	[2, 3]
65	ILSLPELKANPFKER	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8]
70	ELKANPFKERICRVF	ubiquitination	[3, 4, 5, 8]
188	PDFASSFKIVL****	ubiquitination	[3]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[5] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[7] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[8] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]

Functional Description

May convert the inactive conformation of integrin alpha- IIb/beta3 to an active form through binding to the integrin cytoplasmic domain. Induces cell migration and spreading mediated through integrin (possibly via focal adhesion complexes). Functions as a negative regulator of stress activated MAP kinase (MAPK) signaling pathways. May play a role in regulation of apoptosis. Interacts with and up-regulates PTK2/FAK1 activity. Down regulates inositol 1,4,5-trisphosphate receptor-dependent calcium signaling. Participates in endomitotic cell cycle, a form of mitosis in which both karyokinesis and cytokinesis are interrupted and is a hallmark of megakaryocyte differentiation.

DOMAIN 103 138 EF-hand 1.
DOMAIN 148 183 EF-hand 2.
LIPID 2 2 N-myristoyl glycine.

3D-structure; Calcium; Cell membrane; Cell projection; Complete proteome; Cytoplasm; Lipoprotein; Membrane; Metal-binding; Myristate; Nucleus; Polymorphism; Reference proteome; Repeat.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0016324; C:apical plasma membrane; IEA:UniProtKB-SubCell.
GO:0005783; C:endoplasmic reticulum; IMP:HGNC.
GO:0030175; C:filopodium; IEA:UniProtKB-SubCell.
GO:0005654; C:nucleoplasm; IMP:HGNC.
GO:0042383; C:sarcolemma; IDA:BHF-UCL.
GO:0005509; F:calcium ion binding; IMP:HGNC.
GO:0043495; F:protein anchor; IGI:BHF-UCL.
GO:0007155; P:cell adhesion; TAS:ProtInc.
GO:0006302; P:double-strand break repair; TAS:ProtInc.
GO:0007113; P:endomitotic cell cycle; IDA:UniProtKB.
GO:0097191; P:extrinsic apoptotic signaling pathway; TAS:BHF-UCL.
GO:0008285; P:negative regulation of cell proliferation; IMP:UniProtKB.
GO:0070886; P:positive regulation of calcineurin-NFAT signaling cascade; IDA:BHF-UCL.
GO:0090004; P:positive regulation of establishment of protein localization to plasma membrane; IGI:BHF-UCL.

IPR011992; EF-hand-like_dom.
IPR018247; EF_Hand_1_Ca_BS.
IPR002048; EF_hand_dom.

PS00018; EF_HAND_1
PS50222; EF_HAND_2