CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023563
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Cholesterol 24-hydroxylase 
Protein Synonyms/Alias
 CH24H; Cytochrome P450 46A1 
Gene Name
 Cyp46a1 
Gene Synonyms/Alias
 Cyp46 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
69DVFLDWAKKYGPVVRubiquitination[1]
255RLLRQVGKDWVQRRRubiquitination[1]
339VDEVVGSKRHLDYEDubiquitination[1]
358QYLSQVLKESLRLYPubiquitination[1]
422RFGPGAPKPRFTYFPubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Involved in the turnover of cholesterol. It converts cholesterol into 24S-hydroxycholesterol and, to a lesser extent, 25-hydroxycholesterol. Has also activity with xenobiotic compounds. 
Sequence Annotation
 METAL 437 437 Iron (heme axial ligand) (By similarity).  
Keyword
 Cholesterol metabolism; Complete proteome; Endoplasmic reticulum; Heme; Iron; Lipid metabolism; Membrane; Metal-binding; Microsome; Monooxygenase; NADP; Oxidoreductase; Reference proteome; Steroid metabolism; Sterol metabolism; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 500 AA 
Protein Sequence
MSPGLLLLGS AVLLAFGLCC TFVHRARSRY EHIPGPPRPS FLLGHLPYFW KKDEDCGRVL 60
QDVFLDWAKK YGPVVRVNVF YKTSVIVTSP ESVKKFLMST KYNKDSKMYR ALQTVFGERL 120
FGQGLVSECD YGRWYKQRKV MDLAFSRSSL VSLMETFNEK AEQLVEILEA KADGQTPVSM 180
QDMLTCATID ILAKAAFGME TSMLLGAQKP LSQAVKVMLE GISASRNTLA KFMPGKRKQL 240
REIRESIRLL RQVGKDWVQR RREALKRGED MPADILTQIL KAEEGAQDDE VLLDNFVTFF 300
IAGHETSANH LAFTVMELSR QPEIVARLQA EVDEVVGSKR HLDYEDLGRL QYLSQVLKES 360
LRLYPPAWGT FRLLEEETLI DGVRVPGNTP LLFSTYVMGR MDTYFEDPLT FNPDRFGPGA 420
PKPRFTYFPF SLGHRSCIGQ QFAQMEVKVV MAKLLQRIEF RLVPGQRFGL QEQATLKPLD 480
PVLCTLRPRG WQPAPPPPPC 500 
Gene Ontology
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0033781; F:cholesterol 24-hydroxylase activity; ISS:UniProtKB.
 GO:0009055; F:electron carrier activity; IEA:InterPro.
 GO:0020037; F:heme binding; ISS:UniProtKB.
 GO:0005506; F:iron ion binding; IEA:InterPro.
 GO:0006707; P:cholesterol catabolic process; ISS:UniProtKB.
 GO:0006805; P:xenobiotic metabolic process; ISS:UniProtKB. 
Interpro
 IPR001128; Cyt_P450.
 IPR017972; Cyt_P450_CS.
 IPR002401; Cyt_P450_E_grp-I. 
Pfam
 PF00067; p450 
SMART
  
PROSITE
 PS00086; CYTOCHROME_P450 
PRINTS
 PR00463; EP450I.
 PR00385; P450.