UniProt Accession

 ETFB_MOUSE; Q9DCW4; Q810V3 

Genbank Protein ID

 AK002407; AK150293; BC069877; BC049237 

Genbank Nucleotide ID

 BAB22076.1; BAE29447.1; AAH69877.1; AAH49237.1 

Protein Name

 Electron transfer flavoprotein subunit beta 

Protein Synonyms/Alias

 Beta-ETF 

Gene Name

 Etfb 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
19	RVIDFAVKIRVKPDK	acetylation	[1, 2, 3, 4]
19	RVIDFAVKIRVKPDK	succinylation	[3]
26	KIRVKPDKSGVVTDG	acetylation	[4, 5]
35	GVVTDGVKHSMNPFC	acetylation	[2, 3, 4, 5]
35	GVVTDGVKHSMNPFC	succinylation	[3]
56	AVRLKEKKLVKEIIA	acetylation	[1, 2, 5]
59	LKEKKLVKEIIAVSC	acetylation	[2, 3, 4, 5, 6]
59	LKEKKLVKEIIAVSC	succinylation	[3]
110	QVARVLAKLAEKEKV	acetylation	[2, 3, 4, 5, 6, 7, 8]
110	QVARVLAKLAEKEKV	succinylation	[3]
114	VLAKLAEKEKVDLLF	acetylation	[2, 5]
116	AKLAEKEKVDLLFLG	acetylation	[2, 3, 4, 5, 7, 9, 10]
116	AKLAEKEKVDLLFLG	succinylation	[3]
116	AKLAEKEKVDLLFLG	ubiquitination	[11]
124	VDLLFLGKQAIDDDC	acetylation	[4]
176	GLETLRLKLPAVVTA	acetylation	[4, 5, 6]
176	GLETLRLKLPAVVTA	ubiquitination	[11]
200	ATLPNIMKAKKKKIE	acetylation	[2]
202	LPNIMKAKKKKIEVV	acetylation	[2]
203	PNIMKAKKKKIEVVK	acetylation	[2]
204	NIMKAKKKKIEVVKA	acetylation	[2]
205	IMKAKKKKIEVVKAG	acetylation	[1]
210	KKKIEVVKAGDLGVD	acetylation	[2, 3, 4, 5, 6]
210	KKKIEVVKAGDLGVD	succinylation	[3]
210	KKKIEVVKAGDLGVD	ubiquitination	[11]
221	LGVDLTSKVSVISVE	acetylation	[4]
238	PQRSAGVKVETTEDL	acetylation	[2, 3, 5]
238	PQRSAGVKVETTEDL	succinylation	[3]
238	PQRSAGVKVETTEDL	ubiquitination	[11]
248	TTEDLVAKLKEVGRI	acetylation	[2, 3, 4, 5, 6, 8, 9, 10]
248	TTEDLVAKLKEVGRI	succinylation	[3]
248	TTEDLVAKLKEVGRI	ubiquitination	[11]
250	EDLVAKLKEVGRI**	acetylation	[2]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [2] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [3] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [5] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [6] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [7] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [8] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599]
 [9] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199]
 [10] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [11] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 The electron transfer flavoprotein serves as a specific electron acceptor for several dehydrogenases, including five acyl- CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (ETF dehydrogenase) (By similarity). 

Sequence Annotation

 MOD_RES 2 2 N-acetylalanine.  

Keyword

 Acetylation; Complete proteome; Direct protein sequencing; Electron transport; FAD; Flavoprotein; Mitochondrion; Reference proteome; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 255 AA 

Protein Sequence

Gene Ontology

 GO:0017133; C:mitochondrial electron transfer flavoprotein complex; TAS:MGI.
 GO:0009055; F:electron carrier activity; TAS:MGI.
 GO:0022900; P:electron transport chain; IEA:UniProtKB-KW. 

Interpro

 IPR000049; ET-Flavoprotein_bsu_CS.
 IPR014730; ETF_a/b_N.
 IPR012255; ETF_b.
 IPR014729; Rossmann-like_a/b/a_fold. 

Pfam

 PF01012; ETF 

SMART

 SM00893; ETF 

PROSITE

 PS01065; ETF_BETA 

PRINTS