CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-025817
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DEAD box polypeptide 17 isoform p82 variant 
Protein Synonyms/Alias
  
Gene Name
  
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
123GERLRKKKWDLSELPubiquitination[1]
131WDLSELPKFEKNFYVubiquitination[1]
134SELPKFEKNFYVEHPubiquitination[1]
271QVADDYGKCSRLKSTubiquitination[1, 2]
286CIYGGAPKGPQIRDLubiquitination[3]
315IDFLESGKTNLRRCTubiquitination[1]
363MWSATWPKEVRQLAEubiquitination[3]
454AMCIHGDKSQPERDWubiquitination[1]
470LNEFRSGKAPILIATubiquitination[1]
517RTARSTNKGTAYTFFubiquitination[1, 3]
530FFTPGNLKQARELIKubiquitination[1, 3]
549ANQAINPKLMQLVDHubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Helicase; Hydrolase; Nucleotide-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 737 AA 
Protein Sequence
PNLPTGFVAP ILCVLLPSPT REAATVASAT GDSASERESA APAAAPTAEA PPPSVVTRPE 60
PQALPSPAIR APLPDLYPFG TMRGGGFGDR DRDRDRGGFG ARGGGGLPPK KFGNPGERLR 120
KKKWDLSELP KFEKNFYVEH PEVARLTPYE VDELRRKKEI TVRGGDVCPK PVFAFHHANF 180
PQYVMDVLMD QHFTEPTPIQ CQGFPLALSG RDMVGIAQTG SGKTLAYLLP AIVHINHQPY 240
LERGDGPICL VLAPTRELAQ QVQQVADDYG KCSRLKSTCI YGGAPKGPQI RDLERGVEIC 300
IATPGRLIDF LESGKTNLRR CTYLVLDEAD RMLDMGFEPQ IRKIVDQIRP DRQTLMWSAT 360
WPKEVRQLAE DFLRDYTQIN VGNLELSANH NILQIVDVCM ESEKDHKLIQ LMEEIMAEKE 420
NKTIIFVETK RRCDDLTRRM RRDGWPAMCI HGDKSQPERD WVLNEFRSGK APILIATDVA 480
SRGLDVEDVK FVINYDYPNS SEDYVHRIGR TARSTNKGTA YTFFTPGNLK QARELIKVLE 540
EANQAINPKL MQLVDHRGGG GGGGKGGRSR YRTTSSANNP NLMYQDECDR RLRGVKDGGR 600
RDSASYRDRS ETDRAGYANG SGYGSPNSAF GAQAGQYTYG QGTYGAAAYG TSSYTAQEYG 660
AGTYGASSTT STGRSSQSSS QQFSGIGRSG QQPQPLMSQQ FAQPPGATNT LLLLPLLLLH 720
VNETTQVVVT PADLITF 737 
Gene Ontology
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003676; F:nucleic acid binding; IEA:InterPro. 
Interpro
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase.
 IPR000629; RNA-helicase_DEAD-box_CS.
 IPR014014; RNA_helicase_DEAD_Q_motif. 
Pfam
 PF00270; DEAD
 PF00271; Helicase_C 
SMART
 SM00487; DEXDc
 SM00490; HELICc 
PROSITE
 PS00039; DEAD_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER
 PS51195; Q_MOTIF 
PRINTS