CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-022614
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 2-hydroxyacyl-CoA lyase 1 
Protein Synonyms/Alias
 2-hydroxyphytanoyl-CoA lyase; 2-HPCL; Phytanoyl-CoA 2-hydroxylase 2 
Gene Name
 Hacl1 
Gene Synonyms/Alias
 Hpcl; Phyh2 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
21EEQVSGAKVIAQALKubiquitination[1]
139EACRLYTKFSARPSTubiquitination[1]
156LIPFIIEKAVRSSIYubiquitination[1]
217ASVLRDAKQPLLIIGubiquitination[1]
225QPLLIIGKGAAYSHAubiquitination[1]
238HAEDSIRKLVEQCSLubiquitination[1]
254FLPTPMGKGVVPDNHubiquitination[1]
368KSNEAISKELASQKSacetylation[2]
368KSNEAISKELASQKSubiquitination[1]
374SKELASQKSLPMNYYubiquitination[1]
554QALQDTSKPCLLNIMubiquitination[1]
569IEPQSTRKAQDFHWLubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123] 
Functional Description
 Catalyzes a carbon-carbon cleavage reaction; cleaves a 2-hydroxy-3-methylacyl-CoA into formyl-CoA and a 2-methyl-branched fatty aldehyde (By similarity). 
Sequence Annotation
 REGION 404 487 Thiamine pyrophosphate binding.
 MOTIF 579 581 Microbody targeting signal (By
 METAL 458 458 Magnesium (By similarity).
 METAL 485 485 Magnesium (By similarity).
 BINDING 63 63 Thiamine pyrophosphate (By similarity).
 MOD_RES 4 4 Phosphoserine.
 MOD_RES 10 10 Phosphoserine.  
Keyword
 Complete proteome; Lyase; Magnesium; Metal-binding; Peroxisome; Phosphoprotein; Reference proteome; Thiamine pyrophosphate. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 581 AA 
Protein Sequence
MPESNSAEGS DRSEEQVSGA KVIAQALKTQ DVEYMFGVVG IPVTEIALAA QELGIKYIGM 60
RNEQAACYAA SAVGYLTGRP GVCLVVSGPG LIHALGGMAN ANMNCWPLIV IGGSSERNQE 120
AMGAFQEFPQ VEACRLYTKF SARPSTIELI PFIIEKAVRS SIYGRPGACY IDIPADFVTL 180
QANVTSIKYK ECCMPPPVSM AETSAVCAAA SVLRDAKQPL LIIGKGAAYS HAEDSIRKLV 240
EQCSLPFLPT PMGKGVVPDN HPNCVGAARS RALQSADVIV LFGARLNWIL HFGLPPRYQA 300
DVKFIQIDIC AEELGNNVRP SVILLGDIDA VSKQLLEQFD KTPWQCPPDS QWWKTLREKM 360
KSNEAISKEL ASQKSLPMNY YTVFYHVQEQ LPRDSFIVSE GANTMDIGRT MLQNCLPRHR 420
LDAGSFGTMG VGLGFAIAAA LVAKDRSPGQ RVICVEGDSA FGFSGMEVET ICRYNLPIIL 480
LVVNNNGIYQ GFDADTWEKM LHFQEAATTV PPMCLLPNSH YEQVMTAFGG KGYFVRTPEE 540
LQHSLRQALQ DTSKPCLLNI MIEPQSTRKA QDFHWLTRSN M 581 
Gene Ontology
 GO:0005777; C:peroxisome; ISS:HGNC.
 GO:0016830; F:carbon-carbon lyase activity; ISS:HGNC.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0030976; F:thiamine pyrophosphate binding; ISS:HGNC.
 GO:0001561; P:fatty acid alpha-oxidation; ISS:HGNC.
 GO:0051259; P:protein oligomerization; IEA:Compara. 
Interpro
 IPR012000; Thiamin_PyroP_enz_cen_dom.
 IPR012001; Thiamin_PyroP_enz_TPP-bd_dom.
 IPR011766; TPP_enzyme-bd_C. 
Pfam
 PF02775; TPP_enzyme_C
 PF00205; TPP_enzyme_M
 PF02776; TPP_enzyme_N 
SMART
  
PROSITE
 PS00187; TPP_ENZYMES 
PRINTS