CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-013532
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Outer dense fiber protein 2 
Protein Synonyms/Alias
 Cenexin; Outer dense fiber of sperm tails protein 2 
Gene Name
 ODF2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
138MNAVGCLKSEVKMQKubiquitination[1]
190IERMKEEKDFTILQKubiquitination[2, 3]
198DFTILQKKHLQQEKEubiquitination[1]
204KKHLQQEKECLMSKLubiquitination[1]
210EKECLMSKLVEAEMDubiquitination[1]
223MDGAAAAKQVMALKDubiquitination[1]
229AKQVMALKDTIGKLKubiquitination[1]
239IGKLKTEKQMTCTDIubiquitination[1, 4]
253INTLTRQKELLLQKLubiquitination[1, 4]
259QKELLLQKLSTFEETubiquitination[1, 2, 3, 4, 5]
295EQQGALLKRLAEADSubiquitination[1, 2, 3, 4, 6]
368SRLCMQIKNLERSGNubiquitination[1]
389EAIMEQLKELKQKGDubiquitination[1]
505TAEYSAFKLENERLKubiquitination[1]
537QQLKASVKNYEGMIDubiquitination[1]
572ERCDKENKILKDEMNubiquitination[2, 3]
575DKENKILKDEMNKEIacetylation[7]
609ILKITEAKLAECQDQubiquitination[1]
623QLQGYERKNIDLTAIubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [7] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786] 
Functional Description
 Seems to be a major component of sperm tail outer dense fibers (ODF). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail and may help to maintain the passive elastic structures and elastic recoil of the sperm tail. May have a modulating influence on sperm motility. Functions as a general scaffold protein that is specifically localized at the distal/subdistal appendages of mother centrioles. Component of the centrosome matrix required for the localization of PLK1 and NIN to the centrosomes. Required for the formation and/or maintenance of normal CETN1 assembly. 
Sequence Annotation
  
Keyword
 Alternative splicing; Cell projection; Cilium; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Developmental protein; Differentiation; Microtubule; Phosphoprotein; Polymorphism; Reference proteome; Spermatogenesis. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 829 AA 
Protein Sequence
MSASSSGGSP RFPSCGKNGV TSLTQKKVLR APCGAPSVTV TKSHKRGMKG DTVNVRRSVR 60
VKTKVPWMPP GKSSARPVGC KWENPPHCLE ITPPSSEKLV SVMRLSDLST EDDDSGHCKM 120
NRYDKKIDSL MNAVGCLKSE VKMQKGERQM AKRFLEERKE ELEEVAHELA ETEHENTVLR 180
HNIERMKEEK DFTILQKKHL QQEKECLMSK LVEAEMDGAA AAKQVMALKD TIGKLKTEKQ 240
MTCTDINTLT RQKELLLQKL STFEETNRTL RDLLREQHCK EDSERLMEQQ GALLKRLAEA 300
DSEKARLLLL LQDKDKEVEE LLQEIQCEKA QAKTASELSK SMESMRGHLQ AQLRSKEAEN 360
SRLCMQIKNL ERSGNQHKAE VEAIMEQLKE LKQKGDRDKE SLKKAIRAQK ERAEKSEEYA 420
EQLHVQLADK DLYVAEALST LESWRSRYNQ VVKEKGDLEL EIIVLNDRVT DLVNQQQTLE 480
EKMREDRDSL VERLHRQTAE YSAFKLENER LKASFAPMED KLNQAHLEVQ QLKASVKNYE 540
GMIDNYKSQV MKTRLEADEV AAQLERCDKE NKILKDEMNK EIEAARRQFQ SQLADLQQLP 600
DILKITEAKL AECQDQLQGY ERKNIDLTAI ISDLRSRIEH QGDKLEMARE KHQASQKENK 660
QLSLKVDELE RKLEATSAQN IEFLQVIAKR EEAIHQSQLR LEEKTRECGT LARQLESAIE 720
DARRQVEQTK EHALSKERAA QNKILDLETQ LSRTKTELSQ LRRSRDDADR RYQSRLQDLK 780
DRLEQSESTN RSMQNYVQFL KSSYANVFGD GPYSTFLTSS PIRSRSPPA 829 
Gene Ontology
 GO:0005814; C:centriole; IEA:UniProtKB-SubCell.
 GO:0005813; C:centrosome; IDA:UniProtKB.
 GO:0005929; C:cilium; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0001520; C:outer dense fiber; IEA:Compara.
 GO:0000922; C:spindle pole; IEA:UniProtKB-SubCell.
 GO:0005198; F:structural molecule activity; TAS:ProtInc.
 GO:0000086; P:G2/M transition of mitotic cell cycle; TAS:Reactome.
 GO:0007275; P:multicellular organismal development; IEA:UniProtKB-KW.
 GO:0007286; P:spermatid development; IEA:Compara. 
Interpro
 IPR026099; Odf2-rel. 
Pfam
  
SMART
  
PROSITE
  
PRINTS