CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006583
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 ATP-dependent (S)-NAD(P)H-hydrate dehydratase 
Protein Synonyms/Alias
 ATP-dependent NAD(P)HX dehydratase 
Gene Name
 YKL151C; YKL606 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
142IIRYILEKHEGKIPLacetylation[1]
187TPNVVEFKRLCDAIGacetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 Catalyzes the dehydration of the S-form of NAD(P)HX at the expense of ATP, which is converted to ADP. Together with NAD(P)HX epimerase, which catalyzes the epimerization of the S- and R-forms, the enzyme allows the repair of both epimers of NAD(P)HX, a damaged form of NAD(P)H that is a result of enzymatic or heat-dependent hydration. 
Sequence Annotation
 DOMAIN 11 335 YjeF C-terminal.
 NP_BIND 218 222 ATP (By similarity).
 NP_BIND 240 249 ATP (By similarity).
 REGION 182 188 NAD(P)HX (By similarity).
 BINDING 121 121 NAD(P)HX; via amide nitrogen (By
 BINDING 250 250 NAD(P)HX (By similarity).
 MOD_RES 6 6 Phosphoserine.  
Keyword
 ATP-binding; Complete proteome; Cytoplasm; Lyase; NAD; NADP; Nucleotide-binding; Phosphoprotein; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 337 AA 
Protein Sequence
MLAELSHREL IKLAQKRCIP PLLPKFHKGQ SGGRVCIIGG CEDYTGAPYF SANATALMGC 60
DLTHVICEYN AGTVIKSYTP NLMVHPYLRM SNTKLDVDMD EQRKKINSLL DRIHVVVIGP 120
GLGRDPLMLK SIKDIIRYIL EKHEGKIPLV IDADGLFLVT QDSEVKEMLK SYPKGRVILT 180
PNVVEFKRLC DAIGKKGDSH SEMGSLIAQE LNCIVVEKGQ SDKIFSPDSE KDMLTNSEEG 240
SNKRVGGQGD TLTGAISCML AFSRAMYDFK ICEQEEKGES SNDKPLKNWV DYAMLSCYAG 300
CTITRECSRL GFKAKGRAMQ TTDLNDRVGE VFAKLFG 337 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0047453; F:ATP-dependent NAD(P)H-hydrate dehydratase activity; IDA:SGD.
 GO:0046496; P:nicotinamide nucleotide metabolic process; IDA:SGD. 
Interpro
 IPR017953; Carbohydrate_kinase_pred_CS.
 IPR026599; NnrD/CARKD.
 IPR000631; YjeF_C_carb_kinase-rel. 
Pfam
 PF01256; Carb_kinase 
SMART
  
PROSITE
 PS01049; YJEF_C_1
 PS01050; YJEF_C_2
 PS51383; YJEF_C_3 
PRINTS