| Tag | Content |
|---|
| CPLM ID | CPLM-012773 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | tRNA (cytosine(34)-C(5))-methyltransferase |
Protein Synonyms/Alias | Myc-induced SUN domain-containing protein; Misu; NOL1/NOP2/Sun domain family member 2 |
Gene Name | Nsun2 |
Gene Synonyms/Alias | D13Wsu123e; Misu |
Created Date | July 27, 2013 |
Organism | Mus musculus (Mouse) |
NCBI Taxa ID | 10090 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 46 | PEIVKENKLFEHYYQ | ubiquitination | [1] | | 144 | RKSPLLAKFHQFLVS | ubiquitination | [1] | | 365 | MPGVSQWKVMTRDGQ | ubiquitination | [1] | | 639 | QENPFFRKLSSEAYS | ubiquitination | [1] |
|
Reference | [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues. Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C. Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [ PMID: 22790023] |
Functional Description | RNA methyltransferase that methylates tRNAs, and possibly RNA polymerase III transcripts. Methylates cytosine to 5- methylcytosine (m5C) at position 34 of intron-containing tRNA(Leu)(CAA) precursors. Not able to modify tRNAs at positions 48 or 49 (By similarity). May act downstream of Myc to regulate epidermal cell growth and proliferation. Required for proper spindle assembly and chromosome segregation, independently of its methyltransferase activity. |
Sequence Annotation | REGION 184 190 S-adenosyl-L-methionine binding (By
ACT_SITE 321 321 Nucleophile (Potential).
BINDING 215 215 S-adenosyl-L-methionine (By similarity).
BINDING 242 242 S-adenosyl-L-methionine (By similarity).
BINDING 268 268 S-adenosyl-L-methionine (By similarity).
MOD_RES 23 23 Phosphoserine.
MOD_RES 139 139 Phosphoserine; by AURKB (By similarity).
MOD_RES 456 456 Phosphoserine (By similarity).
MOD_RES 473 473 Phosphoserine (By similarity).
MOD_RES 585 585 N6-malonyllysine (By similarity).
MOD_RES 592 592 Phosphoserine (By similarity).
MOD_RES 723 723 Phosphoserine. |
Keyword | Alternative splicing; Cell cycle; Cell division; Complete proteome; Cytoplasm; Cytoskeleton; Methyltransferase; Mitosis; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; S-adenosyl-L-methionine; Transferase; tRNA processing; tRNA-binding. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 757 AA |
Protein Sequence | MGRRARGRRF QQPPQPEGEE DASDGGRKRG QAGWEGGYPE IVKENKLFEH YYQELKIVPE 60
GEWDQFMESL REPLPATLRI TGYKSHAKEI LHCLKNKYFK ELEDLEVDGQ KVEVPQPLSW 120
YPEELAWHTN LSRKILRKSP LLAKFHQFLV SETESGNISR QEAVSMIPPL LLNVEPHHKI 180
LDMCAAPGSK TTQLIEMLHA DMSVPFPEGF VIANDVDNKR CYLLVHQAKR LSSPCIMVVN 240
HDASSIPRLT VDVDGRKEIL FYDRILCDVP CSGDGTMRKN IDVWKKWTTL NSLQLHGLQL 300
RIATRGAEQL AEGGRMVYST CSLNPVEDEA VIAALLEKSE GALELADVSA ELPGLKWMPG 360
VSQWKVMTRD GQWFADWHEV PQGRHTQIRP TMFPPTDLEK LQAMHLERCL RILPHHQNTG 420
GFFVAVLVKK APMPWNKRQP KVQNKSAEAR EPRVSSHVAA TEGNPSDQSE LESQMITGAG 480
DSETAHNTEN TESNEKKDGV CGPPPSKKMK LFGFKEDPFV FIPEDDPLFP PIEKFYALDP 540
SFPRMNLLTR TTEGKKRQLY MVSKELRNVL LNNSEKMKVI NTGIKVWCRN NSGEEFDCAF 600
RLAQEGIYTL YPFINSRIIT VSMEDVKTLL TQENPFFRKL SSEAYSQVKD LAKGSVVLKY 660
EPDSANPDTL QCPIVLCGWR GKASIRTFVP KNERLHYLRM MGLEVLGEKK KEGVILTNEN 720
AASPEQPGDE DAKQTAQDPC VPDSVPGCDA AAAEPSR 757 |
Gene Ontology | |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |