CPLM-000946

Genbank Nucleotide ID

Protein Synonyms/Alias

Homo sapiens (Human)

Position	Peptide	Type	References
14	DTRRLITKPQNLNDA	ubiquitination	[1]
54	TTYEIRVKTNLPIFK	ubiquitination	[1]
61	KTNLPIFKLKESTVR	ubiquitination	[1, 2, 3, 4, 5, 6]
95	VVPPLPGKAFLRQLP	ubiquitination	[1, 2, 4, 5, 6, 7, 8]
119	DNFIEERKQGLEQFI	ubiquitination	[1, 2, 6, 7]
128	GLEQFINKVAGHPLA	ubiquitination	[1]
158	DKSYTPSKIRHA***	ubiquitination	[1, 7]

[1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[4] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
[6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
[8] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]

Functional Description

Phosphoinositide-binding protein required for multivesicular body formation. Specifically binds phosphatidylinositol 3-phosphate (PtdIns(P3)). Plays a role in protein transport between cellular compartments. Promotes stability and cell surface expression of epithelial sodium channel (ENAC) subunits SCNN1A and SCNN1G (By similarity). Not involved in EGFR degradation.

DOMAIN 27 151 PX.
BINDING 70 70 Phosphatidylinositol 3-phosphate (By
BINDING 72 72 Phosphatidylinositol 3-phosphate; via
BINDING 95 95 Phosphatidylinositol 3-phosphate (By
BINDING 118 118 Phosphatidylinositol 3-phosphate (By
MOD_RES 2 2 N-acetylalanine.
MOD_RES 72 72 Phosphoserine.

3D-structure; Acetylation; Alternative splicing; Chromosomal rearrangement; Complete proteome; Direct protein sequencing; Endosome; Lipid-binding; Microphthalmia; Phosphoprotein; Protein transport; Reference proteome; Transport; Ubl conjugation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005769; C:early endosome; IDA:UniProtKB.
GO:0010008; C:endosome membrane; IDA:UniProtKB.
GO:0032266; F:phosphatidylinositol-3-phosphate binding; IDA:UniProtKB.
GO:0007154; P:cell communication; IEA:InterPro.
GO:0006897; P:endocytosis; TAS:ProtInc.
GO:0015031; P:protein transport; IEA:UniProtKB-KW.