| Tag | Content |
|---|
| CPLM ID | CPLM-004075 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | 8-amino-7-oxononanoate synthase |
Protein Synonyms/Alias | AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase |
Gene Name | bioF |
Gene Synonyms/Alias | b0776; JW0759 |
Created Date | July 27, 2013 |
Organism | Escherichia coli (strain K12) |
NCBI Taxa ID | 83333 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 337 | RALQLAEKLRQQGCW | acetylation | [1] |
|
Reference | [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli. Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C. Mol Cell. 2013 Jul 25;51(2):265-72. [ PMID: 23830618] |
Functional Description | Catalyzes the decarboxylative condensation of pimeloyl- [acyl-carrier protein] and L-alanine to produce 8-amino-7- oxononanoate (AON), [acyl-carrier protein], and carbon dioxide. Can also use pimeloyl-CoA instead of pimeloyl-ACP as substrate, but it is believed that pimeloyl-ACP rather than pimeloyl-CoA is the physiological substrate of BioF. |
Sequence Annotation | REGION 108 109 Pyridoxal phosphate binding.
REGION 204 207 Pyridoxal phosphate binding.
REGION 233 236 Pyridoxal phosphate binding.
BINDING 21 21 Substrate.
BINDING 133 133 Substrate.
BINDING 179 179 Pyridoxal phosphate.
BINDING 352 352 Substrate.
MOD_RES 236 236 N6-(pyridoxal phosphate)lysine. |
Keyword | 3D-structure; Biotin biosynthesis; Complete proteome; Direct protein sequencing; Pyridoxal phosphate; Reference proteome; Transferase. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 384 AA |
Protein Sequence | MSWQEKINAA LDARRAADAL RRRYPVAQGA GRWLVADDRQ YLNFSSNDYL GLSHHPQIIR 60
AWQQGAEQFG IGSGGSGHVS GYSVVHQALE EELAEWLGYS RALLFISGFA ANQAVIAAMM 120
AKEDRIAADR LSHASLLEAA SLSPSQLRRF AHNDVTHLAR LLASPCPGQQ MVVTEGVFSM 180
DGDSAPLAEI QQVTQQHNGW LMVDDAHGTG VIGEQGRGSC WLQKVKPELL VVTFGKGFGV 240
SGAAVLCSST VADYLLQFAR HLIYSTSMPP AQAQALRASL AVIRSDEGDA RREKLAALIT 300
RFRAGVQDLP FTLADSCSAI QPLIVGDNSR ALQLAEKLRQ QGCWVTAIRP PTVPAGTARL 360
RLTLTAAHEM QDIDRLLEVL HGNG 384 |
Gene Ontology | GO:0008710; F:8-amino-7-oxononanoate synthase activity; IDA:EcoCyc. GO:0030170; F:pyridoxal phosphate binding; IDA:UniProtKB. GO:0009102; P:biotin biosynthetic process; IMP:EcoCyc. |
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