CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-039850
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 C-1-tetrahydrofolate synthase, cytoplasmic 
Protein Synonyms/Alias
  
Gene Name
 MTHFD1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
66PAEILNGKEISAQIRacetylation[1]
66PAEILNGKEISAQIRubiquitination[2]
77AQIRARLKNQVTQLKubiquitination[2]
84KNQVTQLKEQVPGFTubiquitination[2, 3, 4]
112SNLYINVKLKAAEEIacetylation[1, 4, 5]
114LYINVKLKAAEEIGIubiquitination[2, 4]
122AAEEIGIKATHIKLPubiquitination[2]
127GIKATHIKLPRTTTEubiquitination[2, 4]
206CFIPCTPKGCLELIKubiquitination[2, 4, 6]
213KGCLELIKETGVPIAubiquitination[2]
307DKKPNGRKVVGDVAYubiquitination[2]
318DVAYDEAKERASFITacetylation[5]
318DVAYDEAKERASFITubiquitination[2, 3, 4, 6]
353SAKRFLEKFKPGKWMubiquitination[2]
358LEKFKPGKWMIQYNNubiquitination[2]
369QYNNLNLKTPVPSDIubiquitination[2, 3]
410LYGETKAKVLLSALEubiquitination[2, 7]
426LKHRPDGKYVVVTGIubiquitination[2, 4, 7]
529HELTQTDKALFNRLVacetylation[4, 5]
529HELTQTDKALFNRLVubiquitination[2, 3, 7, 8]
560LKRLGIEKTDPTTLTubiquitination[2, 3]
599TNDRFLRKITIGQAPubiquitination[2, 4, 8, 9]
609IGQAPTEKGHTRTAQacetylation[1, 4, 5]
609IGQAPTEKGHTRTAQubiquitination[2, 4]
644DMRERLGKMVVASSKubiquitination[2, 4, 7, 9]
740MEKFFNIKCRYSGLCubiquitination[2]
777TAGLPLPKAYIQENLubiquitination[2]
789ENLELVEKGFSNLKKubiquitination[2]
796KGFSNLKKQIENARMubiquitination[2]
840HGAFDAVKCTHWAEGubiquitination[2, 4, 7, 9]
849THWAEGGKGALALAQubiquitination[2]
875FQLLYDLKLPVEDKIubiquitination[2, 3, 4, 6]
888KIRIIAQKIYGADDIubiquitination[2]
904LLPEAQHKAEVYTKQubiquitination[3, 4]
910HKAEVYTKQGFGNLPubiquitination[2]
Reference
 [1] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [6] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [7] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [8] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]
 [9] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1020 AA 
Protein Sequence
MRHRVCRGRS GQGRRRSSVI PWPVPKHVGW VVLLGCGGSG TSILVVSIVG SGLIKAMAPA 60
EILNGKEISA QIRARLKNQV TQLKEQVPGF TPRLAILQVG NRDDSNLYIN VKLKAAEEIG 120
IKATHIKLPR TTTESEVMKY ITSLNEDSTV HGFLVQLPLD SENSINTEEV INAIAPEKDV 180
DGLTSINAGK LARGDLNDCF IPCTPKGCLE LIKETGVPIA GRHAVVVGRS KIVGAPMHDL 240
LLWNNATVTT CHSKTAHLDE EVNKGDILVV ATGQPEMVKG EWIKPGAIVI DCGINYVPDD 300
KKPNGRKVVG DVAYDEAKER ASFITPVPGG VGPMTVAMLM QSTVESAKRF LEKFKPGKWM 360
IQYNNLNLKT PVPSDIDISR SCKPKPIGKL AREIGLLSEE VELYGETKAK VLLSALERLK 420
HRPDGKYVVV TGITPTPLGE GKSTTTIGLV QALGAHLYQN VFACVRQPSQ GPTFGIKGGA 480
AGGGYSQVIP MEEFNLHLTG DIHAITAANN LVAAAIDARI FHELTQTDKA LFNRLVPSVN 540
GVRRFSDIQI RRLKRLGIEK TDPTTLTDEE INRFARLDID PETITWQRVL DTNDRFLRKI 600
TIGQAPTEKG HTRTAQFDIS VASEIMAVLA LTTSLEDMRE RLGKMVVASS KKGEPVSAED 660
LGVSGALTVL MKDAIKPNLM QTLEGTPVFV HAGPFANIAH GNSSIIADRI ALKLVGPEGF 720
VVTEAGFGAD IGMEKFFNIK CRYSGLCPHV VVLVATVRAL KMHGGGPTVT AGLPLPKAYI 780
QENLELVEKG FSNLKKQIEN ARMFGIPVVV AVNAFKTDTE SELDLISRLS REHGAFDAVK 840
CTHWAEGGKG ALALAQAVQR AAQAPSSFQL LYDLKLPVED KIRIIAQKIY GADDIELLPE 900
AQHKAEVYTK QGFGNLPICM AKTHLSLSHN PEQKGVPTGF ILPIRDIRAS VGAGFLYPLV 960
GTITIHLQEA TLKVWPVSIQ AHWELGSISK PREVSPCPED LKLIVGVSPE VIFSLNSHHV 1020 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0004329; F:formate-tetrahydrofolate ligase activity; IEA:InterPro.
 GO:0004488; F:methylenetetrahydrofolate dehydrogenase (NADP+) activity; IEA:InterPro.
 GO:0009396; P:folic acid-containing compound biosynthetic process; IEA:InterPro. 
Interpro
 IPR000559; Formate_THF_ligase.
 IPR020628; Formate_THF_ligase_CS.
 IPR016040; NAD(P)-bd_dom.
 IPR027417; P-loop_NTPase.
 IPR000672; THF_DH/CycHdrlase.
 IPR020630; THF_DH/CycHdrlase_cat_dom.
 IPR020867; THF_DH/CycHdrlase_CS.
 IPR020631; THF_DH/CycHdrlase_NAD-bd_dom. 
Pfam
 PF01268; FTHFS
 PF00763; THF_DHG_CYH
 PF02882; THF_DHG_CYH_C 
SMART
  
PROSITE
 PS00721; FTHFS_1
 PS00722; FTHFS_2
 PS00766; THF_DHG_CYH_1
 PS00767; THF_DHG_CYH_2 
PRINTS
 PR00085; THFDHDRGNASE.