CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-005806
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Squalene synthase 
Protein Synonyms/Alias
 SQS; SS; FPP:FPP farnesyltransferase; Farnesyl-diphosphate farnesyltransferase 
Gene Name
 ERG9 
Gene Synonyms/Alias
 YHR190W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
102LLRHFHEKLLLTKWSacetylation[1]
118DGNAPDVKDRAVLTDacetylation[1]
390DKLPPNVKPNETPIFubiquitination[2]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Catalyzes the condensation of 2 two farnesyl pyrophosphate moieties to form squalene. It is the first committed enzyme of the sterol biosynthesis pathway. Required for the biosynthesis of ergosterol. May also have a regulatory role regulating the flux of isoprene intermediates through the sterol pathway. Squalene synthase is crucial for balancing the incorporation of farnesyl diphosphate (FPP) into sterol and nonsterol isoprene synthesis. ERG9 is also essential for cell growth in yeast. 
Sequence Annotation
  
Keyword
 Complete proteome; Endoplasmic reticulum; Isoprene biosynthesis; Lipid biosynthesis; Lipid metabolism; Magnesium; Membrane; Microsome; Multifunctional enzyme; NADP; Oxidoreductase; Reference proteome; Steroid biosynthesis; Steroid metabolism; Sterol biosynthesis; Sterol metabolism; Transferase; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 444 AA 
Protein Sequence
MGKLLQLALH PVEMKAALKL KFCRTPLFSI YDQSTSPYLL HCFELLNLTS RSFAAVIREL 60
HPELRNCVTL FYLILRALDT IEDDMSIEHD LKIDLLRHFH EKLLLTKWSF DGNAPDVKDR 120
AVLTDFESIL IEFHKLKPEY QEVIKEITEK MGNGMADYIL DENYNLNGLQ TVHDYDVYCH 180
YVAGLVGDGL TRLIVIAKFA NESLYSNEQL YESMGLFLQK TNIIRDYNED LVDGRSFWPK 240
EIWSQYAPQL KDFMKPENEQ LGLDCINHLV LNALSHVIDV LTYLAGIHEQ STFQFCAIPQ 300
VMAIATLALV FNNREVLHGN VKIRKGTTCY LILKSRTLRG CVEIFDYYLR DIKSKLAVQD 360
PNFLKLNIQI SKIEQFMEEM YQDKLPPNVK PNETPIFLKV KERSRYDDEL VPTQQEEEYK 420
FNMVLSIILS VLLGFYYIYT LHRA 444 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; IDA:SGD.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IDA:SGD.
 GO:0005741; C:mitochondrial outer membrane; IDA:SGD.
 GO:0004310; F:farnesyl-diphosphate farnesyltransferase activity; IDA:SGD.
 GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
 GO:0051996; F:squalene synthase activity; IDA:SGD.
 GO:0006696; P:ergosterol biosynthetic process; IMP:SGD.
 GO:0008299; P:isoprenoid biosynthetic process; IEA:UniProtKB-KW. 
Interpro
 IPR002060; Squ/phyt_synthse.
 IPR006449; Squal_synth.
 IPR019845; Squalene/phytoene_synthase_CS.
 IPR008949; Terpenoid_synth. 
Pfam
 PF00494; SQS_PSY 
SMART
  
PROSITE
 PS01044; SQUALEN_PHYTOEN_SYN_1
 PS01045; SQUALEN_PHYTOEN_SYN_2 
PRINTS