CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-043459
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 Kinesin-like protein KIF22 
Protein Synonyms/Alias
  
Gene Name
 KIF22 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
19RNHQETLKYQFDAFYubiquitination[1]
65YGPTGAGKTHTMLGSubiquitination[1]
145LIPGLSQKPISSFADubiquitination[1, 2, 3]
201PFRQREGKLYLIDLAubiquitination[1]
224GNKGLRLKESGAINTubiquitination[1]
298LNFAARSKEVINRPFubiquitination[1]
323GPVKLSQKELLGPPEubiquitination[1, 2, 3, 4]
332LLGPPEAKRARGPEEubiquitination[1]
357APASASQKLSPLQKLubiquitination[1, 2]
363QKLSPLQKLSSMDPAubiquitination[1, 4]
397APLLSTPKRERMVLMubiquitination[1, 4]
537LLAHGRQKILDLLNEubiquitination[1, 5]
589RVEGITGKQMESFLKubiquitination[1, 4]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
  
Sequence Annotation
  
Keyword
 ATP-binding; Complete proteome; Microtubule; Motor protein; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 611 AA 
Protein Sequence
MDSCSLEIAN WRNHQETLKY QFDAFYGERS TQQDIYAGSV QPILRHLLEG QNASVLAYGP 60
TGAGKTHTML GSPEQPGVIP RALMDLLQLT REEGAEGRPW ALSVTMSYLE IYQEKVLDLL 120
DPASGDLVIR EDCRGNILIP GLSQKPISSF ADFERHFLPA SRNRTVGATR LNQRSSRSHA 180
VLLVKVDQRE RLAPFRQREG KLYLIDLAGS EDNRRTGNKG LRLKESGAIN TSLFVLGKVV 240
DALNQGLPRV PYRDSKLTRL LQDSLGGSAH SILIANIAPE RRFYLDTVSA LNFAARSKEV 300
INRPFTNESL QPHALGPVKL SQKELLGPPE AKRARGPEEE EIGSPEPMAA PASASQKLSP 360
LQKLSSMDPA MLERLLSLDR LLASQGSQGA PLLSTPKRER MVLMKTVEEK DLEIERLKTK 420
QKELEAKMLA QKAEEKENHC PTMLRPLSHR TVTGAKPLKK AVVMPLQLIQ EQAASPNAEI 480
HILKNKGRKR KVKVAGGLGY TWSPELESLD ALEPEEKAED CWELQISPEL LAHGRQKILD 540
LLNEGSARDL RSLQRIGPKK AQLIVGWREL HGPFSQVEDL ERVEGITGKQ MESFLKANIL 600
GLAAGQRCGA S 611 
Gene Ontology
 GO:0005874; C:microtubule; IEA:UniProtKB-KW.
 GO:0005875; C:microtubule associated complex; IEA:InterPro.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003677; F:DNA binding; IEA:InterPro.
 GO:0003777; F:microtubule motor activity; IEA:InterPro.
 GO:0006281; P:DNA repair; IEA:InterPro.
 GO:0007018; P:microtubule-based movement; IEA:InterPro. 
Interpro
 IPR003583; Hlx-hairpin-Hlx_DNA-bd_motif.
 IPR026986; KIF22.
 IPR019821; Kinesin_motor_CS.
 IPR001752; Kinesin_motor_dom.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00225; Kinesin 
SMART
 SM00278; HhH1
 SM00129; KISc 
PROSITE
 PS00411; KINESIN_MOTOR_DOMAIN1
 PS50067; KINESIN_MOTOR_DOMAIN2 
PRINTS
 PR00380; KINESINHEAVY.