UniProt Accession

 HTRA1_MOUSE; Q9R118; Q8BN04; Q8BN05; Q91WS3; Q9QZK6 

Genbank Protein ID

 AF172994; AF179369; CH466531; BC013516; AK090320; AK090321 

Genbank Nucleotide ID

 AAD49422.1; AAD52682.1; EDL17689.1; AAH13516.1; BAC41168.1; BAC41169.1 

Protein Name

 Serine protease HTRA1 

Protein Synonyms/Alias

 High-temperature requirement A serine peptidase 1; Serine protease 11 

Gene Name

 Htra1 

Gene Synonyms/Alias

 Htra; Prss11 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
373	ESHDRQAKGKAVTKK	acetylation	[1]
375	HDRQAKGKAVTKKKY	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, but it is unclear whether it leads to the proteolytic degradation of TGF-beta proteins themselves (PubMed:18551132) or not (PubMed:14973287). By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets. 

Sequence Annotation

 DOMAIN 33 100 IGFBP N-terminal.
 DOMAIN 98 157 Kazal-like.
 DOMAIN 365 467 PDZ.
 REGION 204 364 Serine protease.
 ACT_SITE 220 220 Charge relay system (Potential).
 ACT_SITE 250 250 Charge relay system (Potential).
 ACT_SITE 328 328 Charge relay system (Potential).  

Keyword

 Complete proteome; Cytoplasm; Growth factor binding; Hydrolase; Protease; Reference proteome; Secreted; Serine protease; Signal. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 480 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0031012; C:extracellular matrix; IEA:Compara.
 GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
 GO:0004252; F:serine-type endopeptidase activity; IEA:InterPro.
 GO:0008236; F:serine-type peptidase activity; IDA:UniProtKB.
 GO:0030514; P:negative regulation of BMP signaling pathway; IDA:MGI.
 GO:0030512; P:negative regulation of transforming growth factor beta receptor signaling pathway; IDA:MGI.
 GO:0006508; P:proteolysis; IDA:UniProtKB.
 GO:0001558; P:regulation of cell growth; IEA:InterPro. 

Interpro

 IPR000867; IGFBP-like.
 IPR002350; Kazal_dom.
 IPR001478; PDZ.
 IPR001940; Peptidase_S1C.
 IPR009003; Trypsin-like_Pept_dom. 

Pfam

 PF00219; IGFBP
 PF07648; Kazal_2
 PF00595; PDZ 

SMART

 SM00121; IB
 SM00280; KAZAL
 SM00228; PDZ 

PROSITE

 PS51323; IGFBP_N_2
 PS51465; KAZAL_2
 PS50106; PDZ 

PRINTS

 PR00834; PROTEASES2C.