CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000196
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Solute carrier family 22 member 1 
Protein Synonyms/Alias
 Organic cation transporter 1; mOCT1 
Gene Name
 Slc22a1 
Gene Synonyms/Alias
 Lx1; Oct1 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
309IMEQIAQKNRKVPPAubiquitination[1]
319KVPPADLKMMCLEEDubiquitination[1]
540GRRKSKAKENTIYLQubiquitination[1]
552YLQVQTGKSPHT***ubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 Translocates a broad array of organic cations with various structures and molecular weights including the model compounds 1-methyl-4-phenylpyridinium (MPP), tetraethylammonium (TEA), N-1-methylnicotinamide (NMN), 4-(4-(dimethylamino)styryl)- N-methylpyridinium (ASP), the endogenous compounds choline, guanidine, histamine, epinephrine, adrenaline, noradrenaline and dopamine, and the drugs quinine, and metformin. The transport of organic cations is inhibited by a broad array of compounds like tetramethylammonium (TMA), cocaine, lidocaine, NMDA receptor antagonists, atropine, prazosin, cimetidine, TEA and NMN, guanidine, cimetidine, choline, procainamide, quinine, tetrabutylammonium, and tetrapentylammonium. Translocates organic cations in an electrogenic and pH-independent manner. Translocates organic cations across the plasma membrane in both directions. Transports the polyamines spermine and spermidine. Transports pramipexole across the basolateral membrane of the proximal tubular epithelial cells. The choline transport is activated by MMTS. Regulated by various intracellular signaling pathways including inhibition by protein kinase A activation, and endogenously activation by the calmodulin complex, the calmodulin- dependent kinase II and LCK tyrosine kinase. 
Sequence Annotation
 CARBOHYD 71 71 N-linked (GlcNAc...) (Potential).  
Keyword
 Cell membrane; Complete proteome; Glycoprotein; Ion transport; Membrane; Phosphoprotein; Reference proteome; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 556 AA 
Protein Sequence
MPTVDDVLEH VGEFGWFQKQ AFLLLCLISA SLAPIYVGIV FLGFTPDHHC RSPGVAELSQ 60
RCGWSPAEEL NYTVPGLGSA GEASFLSQCM KYEVDWNQST LDCVDPLSSL AANRSHLPLS 120
PCEHGWVYDT PGSSIVTEFN LVCGDAWKVD LFQSCVNLGF FLGSLVVGYI ADRFGRKLCL 180
LVTTLVTSLS GVLTAVAPDY TSMLLFRLLQ GMVSKGSWVS GYTLITEFVG SGYRRTTAIL 240
YQVAFTVGLV GLAGVAYAIP DWRWLQLAVS LPTFLFLLYY WFVPESPRWL LSQKRTTQAV 300
RIMEQIAQKN RKVPPADLKM MCLEEDASER RSPSFADLFR TPSLRKHTLI LMYLWFSCAV 360
LYQGLIMHVG ATGANLYLDF FYSSLVEFPA AFIILVTIDR IGRIYPIAAS NLVAGAACLL 420
MIFIPHELHW LNVTLACLGR MGATIVLQMV CLVNAELYPT FIRNLGMMVC SALCDLGGIF 480
TPFMVFRLME VWQALPLILF GVLGLSAGAV TLLLPETKGV ALPETIEEAE NLGRRKSKAK 540
ENTIYLQVQT GKSPHT 556 
Gene Ontology
 GO:0016323; C:basolateral plasma membrane; IEA:UniProtKB-SubCell.
 GO:0005887; C:integral to plasma membrane; ISA:MGI.
 GO:0005277; F:acetylcholine transmembrane transporter activity; IEA:Compara.
 GO:0005329; F:dopamine transmembrane transporter activity; IEA:Compara.
 GO:0005333; F:norepinephrine transmembrane transporter activity; IEA:Compara.
 GO:0015101; F:organic cation transmembrane transporter activity; IDA:MGI.
 GO:0008513; F:secondary active organic cation transmembrane transporter activity; IEA:Compara.
 GO:0015872; P:dopamine transport; IEA:Compara.
 GO:0006855; P:drug transmembrane transport; IEA:Compara.
 GO:0048241; P:epinephrine transport; IEA:Compara.
 GO:0010248; P:establishment or maintenance of transmembrane electrochemical gradient; IEA:Compara.
 GO:0015874; P:norepinephrine transport; IEA:Compara. 
Interpro
 IPR020846; MFS_dom.
 IPR016196; MFS_dom_general_subst_transpt.
 IPR004749; Orgcat_transp.
 IPR005828; Sub_transporter.
 IPR005829; Sugar_transporter_CS. 
Pfam
 PF00083; Sugar_tr 
SMART
  
PROSITE
 PS50850; MFS
 PS00216; SUGAR_TRANSPORT_1 
PRINTS