| Tag | Content |
|---|
| CPLM ID | CPLM-014460 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Hyaluronidase PH-20 |
Protein Synonyms/Alias | Hyal-PH20; Hyaluronoglucosaminidase PH-20; Sperm adhesion molecule 1; Sperm surface antigen 2B1; Sperm surface protein PH-20 |
Gene Name | Spam1 |
Gene Synonyms/Alias | Ph20; Spam |
Created Date | July 27, 2013 |
Organism | Rattus norvegicus (Rat) |
NCBI Taxa ID | 10116 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 265 | LYPSVYLKKDLKSSR | acetylation | [1] |
|
Reference | [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns. Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV. Cell Rep. 2012 Aug 30;2(2):419-31. [ PMID: 22902405] |
Functional Description | Involved in sperm-egg adhesion. Upon fertilization sperm must first penetrate a layer of cumulus cells that surrounds the egg before reaching the zona pellucida. The cumulus cells are embedded in a matrix containing hyaluronic acid which is formed prior to ovulation. This protein aids in penetrating the layer of cumulus cells by digesting hyaluronic acid (By similarity). |
Sequence Annotation | ACT_SITE 147 147 Proton donor (By similarity).
CARBOHYD 63 63 N-linked (GlcNAc...) (Potential).
CARBOHYD 165 165 N-linked (GlcNAc...) (Potential).
CARBOHYD 179 179 N-linked (GlcNAc...) (Potential).
CARBOHYD 368 368 N-linked (GlcNAc...) (Potential).
CARBOHYD 408 408 N-linked (GlcNAc...) (Potential).
DISULFID 60 351 By similarity.
DISULFID 223 237 By similarity.
DISULFID 376 387 By similarity.
DISULFID 381 435 By similarity.
DISULFID 437 464 By similarity. |
Keyword | Cell adhesion; Cell membrane; Complete proteome; Disulfide bond; Glycoprotein; Glycosidase; GPI-anchor; Hydrolase; Lipoprotein; Membrane; Reference proteome; Signal. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 512 AA |
Protein Sequence | MGELQFKWLF WRSFAESGGT FQTVLIFLFI PYSLTVDYRA TPVLSDTTFV WVWNVPTEAC 60
VENVTEPIDL SFFSLIGSPR KTAIGQPVTL FYVDRLGNYP HIDAQQTEHH GGIPQKGDLT 120
THLVKAKEDV ERYIPTDKLG LAIIDWEEWR PTWMRNWTPK DIYRNKSIEL VQAADPAINI 180
TEATVRAKAQ FEGAAKEFME GTLKLGKHIR PKHLWGFYLF PDCYNNKFQV DNYDGQCPDV 240
EKKRNDDLDW LWKESTGLYP SVYLKKDLKS SRKATLYVRY RVLESIRVSK VSDESNPVPI 300
FVYIRLVFTD HVSEYLLEDD LVNTIGEIVA QGTSGIIIWD AMSLAQRSAG CPILRQYMKT 360
TLNPYIVNVT LAAKMCSQTL CKEKGMCSRK TESSDAYLHL DPSSFSINVT EAGKYEVLGK 420
PEVKDLEYFS EHFKCSCFSK MTCEETSDMR SIQDVNVCMG DNVCIKATLG PNSAFHLLPG 480
KGLLLMTTLA HILHHLPHDI FVFPWKMLVS TP 512 |
Gene Ontology | GO:0001669; C:acrosomal vesicle; IEA:Compara. GO:0031225; C:anchored to membrane; IEA:UniProtKB-KW. GO:0009897; C:external side of plasma membrane; IEA:Compara. GO:0045121; C:membrane raft; IEA:Compara. GO:0004415; F:hyalurononglucosaminidase activity; IEA:EC. GO:0005975; P:carbohydrate metabolic process; IEA:InterPro. GO:0007155; P:cell adhesion; TAS:RGD. GO:0007342; P:fusion of sperm to egg plasma membrane; TAS:RGD. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |