UniProt Accession

 DLDH_YEAST; P09624; D6VTL1 

Genbank Protein ID

 J03645; M20880; D50617; Z46255; BK006940 

Genbank Nucleotide ID

 AAA34565.1; AAB63974.1; BAA09220.1; CAA86354.1; DAA12421.1 

Protein Name

 Dihydrolipoyl dehydrogenase, mitochondrial 

Protein Synonyms/Alias

 Dihydrolipoamide dehydrogenase; Glycine decarboxylase complex subunit L; Lipoamide dehydrogenase component of pyruvate dehydrogenase complex; Pyruvate dehydrogenase complex E3 component 

Gene Name

 LPD1 

Gene Synonyms/Alias

 DHLP1; YFL018C 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
57	FNTACVEKRGKLGGT	acetylation	[1]
110	INVANFQKAKDDAVK	acetylation	[1]
136	KNKVTYYKGNGSFED	acetylation	[1]
146	GSFEDETKIRVTPVD	acetylation	[1]
160	DGLEGTVKEDHILDV	acetylation	[1]
201	STGALSLKEIPKRLT	acetylation	[1]
249	SMDGEVAKATQKFLK	acetylation	[1]
253	EVAKATQKFLKKQGL	acetylation	[1]
324	KIGLEVDKRGRLVID	acetylation	[1]
337	IDDQFNSKFPHIKVV	acetylation	[1]
401	GKTEEQLKEAGIDYK	acetylation	[1]
411	GIDYKIGKFPFAANS	acetylation	[1]
437	VKILIDSKTERILGA	acetylation	[1]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 

Functional Description

 Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes. This includes the pyruvate dehydrogenase complex, which catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). Acts also as component of the glycine cleavage system (glycine decarboxylase complex), which catalyzes the degradation of glycine. 

Sequence Annotation

 NP_BIND 56 65 FAD.
 NP_BIND 174 176 FAD (By similarity).
 NP_BIND 211 218 NAD (By similarity).
 NP_BIND 352 355 FAD.
 ACT_SITE 478 478 Proton acceptor.
 BINDING 74 74 FAD.
 BINDING 139 139 FAD; via amide nitrogen and carbonyl
 BINDING 234 234 NAD (By similarity).
 BINDING 268 268 NAD; via amide nitrogen and carbonyl
 BINDING 305 305 NAD; via amide nitrogen (By similarity).
 BINDING 346 346 FAD.
 DISULFID 65 70 Redox-active.  

Keyword

 3D-structure; Complete proteome; Disulfide bond; FAD; Flavoprotein; Mitochondrion; NAD; Oxidoreductase; Redox-active center; Reference proteome; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 499 AA 

Protein Sequence

Gene Ontology

 GO:0005960; C:glycine cleavage complex; IMP:SGD.
 GO:0042645; C:mitochondrial nucleoid; IDA:SGD.
 GO:0009353; C:mitochondrial oxoglutarate dehydrogenase complex; IDA:SGD.
 GO:0004148; F:dihydrolipoyl dehydrogenase activity; IDA:SGD.
 GO:0050660; F:flavin adenine dinucleotide binding; IEA:InterPro.
 GO:0004375; F:glycine dehydrogenase (decarboxylating) activity; IMP:SGD.
 GO:0004591; F:oxoglutarate dehydrogenase (succinyl-transferring) activity; IMP:SGD.
 GO:0004738; F:pyruvate dehydrogenase activity; IMP:SGD.
 GO:0006103; P:2-oxoglutarate metabolic process; IMP:SGD.
 GO:0045454; P:cell redox homeostasis; IEA:InterPro.
 GO:0006546; P:glycine catabolic process; IMP:SGD.
 GO:0042743; P:hydrogen peroxide metabolic process; IMP:SGD.
 GO:0006550; P:isoleucine catabolic process; IMP:SGD.
 GO:0006564; P:L-serine biosynthetic process; IMP:SGD.
 GO:0006552; P:leucine catabolic process; IMP:SGD.
 GO:0006090; P:pyruvate metabolic process; IMP:SGD.
 GO:0006574; P:valine catabolic process; IMP:SGD. 

Interpro

 IPR016156; FAD/NAD-linked_Rdtase_dimer.
 IPR013027; FAD_pyr_nucl-diS_OxRdtase.
 IPR006258; Lipoamide_DH.
 IPR004099; Pyr_nucl-diS_OxRdtase_dimer.
 IPR023753; Pyr_nucl-diS_OxRdtase_FAD/NAD.
 IPR012999; Pyr_OxRdtase_I_AS.
 IPR001327; Pyr_OxRdtase_NAD-bd_dom. 

Pfam

 PF00070; Pyr_redox
 PF07992; Pyr_redox_2
 PF02852; Pyr_redox_dim 

SMART

  

PROSITE

 PS00076; PYRIDINE_REDOX_1 

PRINTS

 PR00368; FADPNR.