UniProt Accession

 CRBN_HUMAN; Q96SW2; B2R6H4; C9IZA9; C9JAH6; Q6AI62; Q6NVZ0; Q9UHW4 

Genbank Protein ID

 AK027507; AK312577; AC024060; BC017419; BC067811; AF117230; CR627060 

Genbank Nucleotide ID

 BAB55162.1; BAG35471.1; AAH17419.1; AAH67811.1; AAF17211.1; CAH10361.1 

Protein Name

 Protein cereblon 

Protein Synonyms/Alias

  

Gene Name

 CRBN 

Gene Synonyms/Alias

 AD-006 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
43	QDSKEAKKPNIINFD	ubiquitination	[1, 2, 3]
116	MVRNLIQKDRTFAVL	ubiquitination	[2]
166	AIGRQRFKVLELRTQ	ubiquitination	[1, 2, 3]
211	KCQIFPSKPVSREDQ	ubiquitination	[2]
222	REDQCSYKWWQKYQK	ubiquitination	[2]
226	CSYKWWQKYQKRKFH	ubiquitination	[2]
269	REWDENLKDDSLPSN	ubiquitination	[4]
300	VLRIQLLKIGSAIQR	ubiquitination	[1, 2, 3, 5]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 

Functional Description

 Component of some DCX (DDB1-CUL4-X-box) E3 protein ligase complex, a complex that mediates the ubiquitination and subsequent proteasomal degradation of target proteins and is required for limb outgrowth and expression of the fibroblast growth factor FGF8. In the complex, may act as a substrate receptor. Regulates the assembly and neuronal surface expression of large-conductance calcium-activated potassium channels in brain regions involved in memory and learning via its interaction with KCNT1. 

Sequence Annotation

 DOMAIN 80 317 Lon.
 REGION 339 442 Thalidomide-binding.  

Keyword

 Alternative splicing; Complete proteome; Cytoplasm; Membrane; Mental retardation; Nucleus; Reference proteome; Ubl conjugation; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 442 AA 

Protein Sequence

Gene Ontology

 GO:0031464; C:Cul4A-RING ubiquitin ligase complex; IDA:UniProtKB.
 GO:0005737; C:cytoplasm; IDA:UniProtKB.
 GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0004176; F:ATP-dependent peptidase activity; IEA:InterPro.
 GO:0034766; P:negative regulation of ion transmembrane transport; IEA:Compara.
 GO:0032463; P:negative regulation of protein homooligomerization; IEA:Compara.
 GO:0090073; P:positive regulation of protein homodimerization activity; IEA:Compara.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IMP:UniProtKB.
 GO:0016567; P:protein ubiquitination; IMP:UniProtKB. 

Interpro

 IPR003111; Pept_S16_N.
 IPR015947; PUA-like_domain. 

Pfam

 PF02190; LON 

SMART

 SM00464; LON 

PROSITE

  

PRINTS