UniProt Accession

 ENOPH_HUMAN; Q9UHY7; Q7Z4C5; Q9BVC2 

Genbank Protein ID

 AF113125; AK022656; CR457141; BC001317; BC065815; AF177286 

Genbank Nucleotide ID

 AAF14866.1; BAB14160.1; CAG33422.1; AAH01317.1; AAH65815.1; AAQ13671.1 

Protein Name

 Enolase-phosphatase E1 

Protein Synonyms/Alias

 2,3-diketo-5-methylthio-1-phosphopentane phosphatase; MASA homolog 

Gene Name

 ENOPH1 

Gene Synonyms/Alias

 MASA; MSTP145 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
111	DRKTTALKQLQGHMW	ubiquitination	[1]
187	FDTKIGHKVESESYR	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK- MTPene). 

Sequence Annotation

 REGION 153 154 Substrate binding.
 METAL 16 16 Magnesium.
 METAL 18 18 Magnesium; via carbonyl oxygen.
 METAL 212 212 Magnesium.
 BINDING 187 187 Substrate.  

Keyword

 3D-structure; Alternative splicing; Amino-acid biosynthesis; Complete proteome; Cytoplasm; Hydrolase; Magnesium; Metal-binding; Methionine biosynthesis; Nucleus; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 261 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0043874; F:acireductone synthase activity; IDA:UniProtKB.
 GO:0000287; F:magnesium ion binding; IEA:InterPro.
 GO:0008967; F:phosphoglycolate phosphatase activity; IEA:InterPro.
 GO:0019509; P:L-methionine salvage from methylthioadenosine; IDA:UniProtKB.
 GO:0006595; P:polyamine metabolic process; TAS:Reactome. 

Interpro

 IPR023943; Enolase-ppase_E1.
 IPR027511; ENOPH1_eukaryotes.
 IPR023214; HAD-like_dom.
 IPR006439; HAD-SF_hydro_IA_v1. 

Pfam

 PF13419; HAD_2 

SMART

  

PROSITE

  

PRINTS