CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010284
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 NADP-dependent malic enzyme 
Protein Synonyms/Alias
 NADP-ME 
Gene Name
 maeB 
Gene Synonyms/Alias
 ypfF; b2463; JW2447 
Created Date
 July 27, 2013 
Organism
 Escherichia coli (strain K12) 
NCBI Taxa ID
 83333 
Lysine Modification
Position
Peptide
Type
References
27KIQVSPTKPLATQRDacetylation[1]
52APCLEIEKDPLKAYKacetylation[1, 2]
56EIEKDPLKAYKYTARacetylation[1, 3]
59KDPLKAYKYTARGNLacetylation[1, 2]
149ECFYIEQKLRERMNIacetylation[1]
222NIVVCDSKGVIYQGRacetylation[1]
237EPNMAETKAAYAVVDacetylation[1]
247YAVVDDGKRTLDDVIacetylation[1]
275VLTQEMVKKMARAPMacetylation[1, 2]
344TAINEEMKLAAVRAIacetylation[1]
391FDPRLIVKIAPAVAKacetylation[1]
432YKTNLFMKPIFSQARacetylation[1, 2]
469LVTLGLAKPILIGRPacetylation[1]
485VIEMRIQKLGLQIKAacetylation[1, 2]
519TEYFQIMKRRGVTQEacetylation[1]
Reference
 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [3] Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.
 Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y.
 Mol Cell Proteomics. 2009 Feb;8(2):215-25. [PMID: 18723842] 
Functional Description
  
Sequence Annotation
 NP_BIND 76 83 NADP (By similarity).
 REGION 1 428 Malic enzyme.
 REGION 429 759 Phosphate acetyltransferase; required for
 ACT_SITE 94 94 Proton acceptor (By similarity).
 METAL 136 136 Divalent metal cation (By similarity).
 METAL 137 137 Divalent metal cation (By similarity).
 BINDING 162 162 NAD (By similarity).
 BINDING 288 288 NAD (By similarity).
 MOD_RES 56 56 N6-acetyllysine.  
Keyword
 Acetylation; Complete proteome; Metal-binding; Multifunctional enzyme; NADP; Oxidoreductase; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 759 AA 
Protein Sequence
MDDQLKQSAL DFHEFPVPGK IQVSPTKPLA TQRDLALAYS PGVAAPCLEI EKDPLKAYKY 60
TARGNLVAVI SNGTAVLGLG NIGALAGKPV MEGKGVLFKK FAGIDVFDIE VDELDPDKFI 120
EVVAALEPTF GGINLEDIKA PECFYIEQKL RERMNIPVFH DDQHGTAIIS TAAILNGLRV 180
VEKNISDVRM VVSGAGAAAI ACMNLLVALG LQKHNIVVCD SKGVIYQGRE PNMAETKAAY 240
AVVDDGKRTL DDVIEGADIF LGCSGPKVLT QEMVKKMARA PMILALANPE PEILPPLAKE 300
VRPDAIICTG RSDYPNQVNN VLCFPFIFRG ALDVGATAIN EEMKLAAVRA IAELAHAEQS 360
EVVASAYGDQ DLSFGPEYII PKPFDPRLIV KIAPAVAKAA MESGVATRPI ADFDVYIDKL 420
TEFVYKTNLF MKPIFSQARK APKRVVLPEG EEARVLHATQ ELVTLGLAKP ILIGRPNVIE 480
MRIQKLGLQI KAGVDFEIVN NESDPRFKEY WTEYFQIMKR RGVTQEQAQR ALISNPTVIG 540
AIMVQRGEAD AMICGTVGDY HEHFSVVKNV FGYRDGVHTA GAMNALLLPS GNTFIADTYV 600
NDEPDAEELA EITLMAAETV RRFGIEPRVA LLSHSNFGSS DCPSSSKMRQ ALELVRERAP 660
ELMIDGEMHG DAALVEAIRN DRMPDSSLKG SANILVMPNM EAARISYNLL RVSSSEGVTV 720
GPVLMGVAKP VHVLTPIASV RRIVNMVALA VVEAQTQPL 759 
Gene Ontology
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0004473; F:malate dehydrogenase (oxaloacetate-decarboxylating) (NADP+) activity; IDA:EcoCyc.
 GO:0030145; F:manganese ion binding; IDA:EcoCyc.
 GO:0051287; F:NAD binding; IEA:InterPro.
 GO:0016746; F:transferase activity, transferring acyl groups; IEA:InterPro.
 GO:0006108; P:malate metabolic process; IEA:InterPro. 
Interpro
 IPR015884; Malic_enzyme_CS.
 IPR012301; Malic_N.
 IPR012302; Malic_NAD-bd.
 IPR012188; ME_PTA.
 IPR016040; NAD(P)-bd_dom.
 IPR002505; PTA_PTB. 
Pfam
 PF00390; malic
 PF03949; Malic_M
 PF01515; PTA_PTB 
SMART
 SM00919; Malic_M 
PROSITE
 PS00331; MALIC_ENZYMES 
PRINTS