UniProt Accession

 CARA_ECOLI; P0A6F1; P00907 

Genbank Protein ID

 J01597; U00096; AP009048; X70017 

Genbank Nucleotide ID

 AAA23538.1; AAC73143.1; BAB96601.1; CAA49615.1 

Protein Name

 Carbamoyl-phosphate synthase small chain 

Protein Synonyms/Alias

 Carbamoyl-phosphate synthetase glutamine chain 

Gene Name

 carA 

Gene Synonyms/Alias

 pyrA; b0032; JW0030 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
103	EDLSSYLKRHNIVAI	acetylation	[1]
146	DAALALEKARAFPGL	acetylation	[1]
186	GGLPEAKKEDELPFH	acetylation	[1]
230	TSAEDVLKMNPDGIF	acetylation	[1]
302	HPVKDVEKNVVMITA	acetylation	[1]
330	ANLRVTHKSLFDGTL	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

  

Sequence Annotation

 DOMAIN 193 380 Glutamine amidotransferase type-1.
 REGION 1 189 CPSase.
 ACT_SITE 269 269 Nucleophile (By similarity).
 ACT_SITE 353 353 By similarity.
 ACT_SITE 355 355 By similarity.  

Keyword

 3D-structure; Amino-acid biosynthesis; Arginine biosynthesis; ATP-binding; Complete proteome; Direct protein sequencing; Glutamine amidotransferase; Ligase; Nucleotide-binding; Pyrimidine biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 382 AA 

Protein Sequence

Gene Ontology

 GO:0005951; C:carbamoyl-phosphate synthase complex; IDA:EcoCyc.
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005524; F:ATP binding; IEA:HAMAP.
 GO:0004088; F:carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity; IEA:HAMAP.
 GO:0006207; P:'de novo' pyrimidine nucleobase biosynthetic process; IMP:EcoliWiki.
 GO:0044205; P:'de novo' UMP biosynthetic process; IEA:UniProtKB-UniPathway.
 GO:0006526; P:arginine biosynthetic process; IMP:EcoliWiki.
 GO:0070409; P:carbamoyl phosphate biosynthetic process; IEA:InterPro.
 GO:0006543; P:glutamine catabolic process; IEA:InterPro.
 GO:0006541; P:glutamine metabolic process; IMP:EcoliWiki. 

Interpro

 IPR006274; CarbamoylP_synth_ssu.
 IPR002474; CarbamoylP_synth_ssu_N.
 IPR017926; GATASE. 

Pfam

 PF00988; CPSase_sm_chain
 PF00117; GATase 

SMART

 SM01097; CPSase_sm_chain 

PROSITE

 PS51273; GATASE_TYPE_1 

PRINTS