UniProt Accession

 RL5_ECOLI; P62399; P02389; Q2M6X3 

Genbank Protein ID

 X01563; U18997; U00096; AP009048; M10195 

Genbank Nucleotide ID

 CAA25717.1; AAA58105.1; AAC76333.1; BAE77983.1; AAA24051.1 

Protein Name

 50S ribosomal protein L5 

Protein Synonyms/Alias

  

Gene Name

 rplE 

Gene Synonyms/Alias

 b3308; JW3270 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
3	*****MAKLHDYYKD	acetylation	[1, 2, 3]
9	AKLHDYYKDEVVKKL	acetylation	[3, 4]
14	YYKDEVVKKLMTEFN	acetylation	[3]
15	YKDEVVKKLMTEFNY	acetylation	[3]
47	VGEAIADKKLLDNAA	acetylation	[3]
48	GEAIADKKLLDNAAA	acetylation	[2, 3]
64	LAAISGQKPLITKAR	acetylation	[3]
69	GQKPLITKARKSVAG	acetylation	[3]
72	PLITKARKSVAGFKI	acetylation	[3]
78	RKSVAGFKIRQGYPI	acetylation	[2, 3]
88	QGYPIGCKVTLRGER	acetylation	[3]
120	DFRGLSAKSFDGRGN	acetylation	[2, 3, 4]
145	FPEIDYDKVDRVRGL	acetylation	[3]
161	ITITTTAKSDEEGRA	acetylation	[3]

Reference

 [1] Lysine acetylation is a highly abundant and evolutionarily conserved modification in Escherichia coli.
 Zhang J, Sprung R, Pei J, Tan X, Kim S, Zhu H, Liu CF, Grishin NV, Zhao Y.
 Mol Cell Proteomics. 2009 Feb;8(2):215-25. [PMID: 18723842]
 [2] Comprehensive profiling of protein lysine acetylation in Escherichia coli.
 Zhang K, Zheng S, Yang JS, Chen Y, Cheng Z.
 J Proteome Res. 2013 Feb 1;12(2):844-51. [PMID: 23294111]
 [3] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618]
 [4] The diversity of lysine-acetylated proteins in Escherichia coli.
 Yu BJ, Kim JA, Moon JH, Ryu SE, Pan JG.
 J Microbiol Biotechnol. 2008 Sep;18(9):1529-36. [PMID: 18852508] 

Functional Description

 This is 1 of the proteins that binds and probably mediates the attachment of the 5S RNA into the large ribosomal subunit, where it forms part of the central protuberance. Its 5S rRNA binding is significantly enhanced in the presence of L18. 

Sequence Annotation

 MOD_RES 3 3 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Complete proteome; Direct protein sequencing; Reference proteome; Ribonucleoprotein; Ribosomal protein; RNA-binding; rRNA-binding; tRNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 179 AA 

Protein Sequence

Gene Ontology

 GO:0005840; C:ribosome; IEA:UniProtKB-KW.
 GO:0019843; F:rRNA binding; IEA:HAMAP.
 GO:0003735; F:structural constituent of ribosome; IEA:InterPro.
 GO:0000049; F:tRNA binding; IEA:HAMAP.
 GO:0006412; P:translation; IEA:HAMAP. 

Interpro

 IPR002132; Ribosomal_L5.
 IPR020930; Ribosomal_L5_bac-type.
 IPR020929; Ribosomal_L5_CS.
 IPR022803; Ribosomal_L5_domain. 

Pfam

 PF00281; Ribosomal_L5
 PF00673; Ribosomal_L5_C 

SMART

  

PROSITE

 PS00358; RIBOSOMAL_L5 

PRINTS