CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000743
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Pleiotropic regulator 1 
Protein Synonyms/Alias
  
Gene Name
 PLRG1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
31MFVADNGKPVPLDEEubiquitination[1]
62VLHMPTSKENLKEKGubiquitination[1]
68SKENLKEKGPQNATDubiquitination[1]
80ATDSYVHKQYPANQGubiquitination[1]
113VALTADTKIQRMPSEubiquitination[1]
135VALPLQTKADANRTAubiquitination[1]
173VMETGNTKNSALMAKubiquitination[1]
229GSADRTIKIWDLASGubiquitination[1]
237IWDLASGKLKLSLTGubiquitination[1, 2]
320RIWDVRTKASVHTLSubiquitination[1]
363LWDLVAGKTRVTLTNubiquitination[1]
396SGSPDNIKQWKFPDGubiquitination[1, 2]
505ETHPVSWKPEIIKRKubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 Component of the PRP19-CDC5L complex that forms an integral part of the spliceosome and is required for activating pre-mRNA splicing (By similarity). 
Sequence Annotation
 REPEAT 202 241 WD 1.
 REPEAT 244 283 WD 2.
 REPEAT 286 325 WD 3.
 REPEAT 328 367 WD 4.
 REPEAT 370 410 WD 5.
 REPEAT 411 449 WD 6.
 REPEAT 460 499 WD 7.
 MOD_RES 1 1 N-acetylmethionine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Direct protein sequencing; mRNA processing; mRNA splicing; Nucleus; Reference proteome; Repeat; Spliceosome; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 514 AA 
Protein Sequence
MVEEVQKHSV HTLVFRSLKR THDMFVADNG KPVPLDEESH KRKMAIKLRN EYGPVLHMPT 60
SKENLKEKGP QNATDSYVHK QYPANQGQEV EYFVAGTHPY PPGPGVALTA DTKIQRMPSE 120
SAAQSLAVAL PLQTKADANR TAPSGSEYRH PGASDRPQPT AMNSIVMETG NTKNSALMAK 180
KAPTMPKPQW HPPWKLYRVI SGHLGWVRCI AVEPGNQWFV TGSADRTIKI WDLASGKLKL 240
SLTGHISTVR GVIVSTRSPY LFSCGEDKQV KCWDLEYNKV IRHYHGHLSA VYGLDLHPTI 300
DVLVTCSRDS TARIWDVRTK ASVHTLSGHT NAVATVRCQA AEPQIITGSH DTTIRLWDLV 360
AGKTRVTLTN HKKSVRAVVL HPRHYTFASG SPDNIKQWKF PDGSFIQNLS GHNAIINTLT 420
VNSDGVLVSG ADNGTMHLWD WRTGYNFQRV HAAVQPGSLD SESGIFACAF DQSESRLLTA 480
EADKTIKVYR EDDTATEETH PVSWKPEIIK RKRF 514 
Gene Ontology
 GO:0071013; C:catalytic step 2 spliceosome; IDA:UniProtKB.
 GO:0016607; C:nuclear speck; IEA:UniProtKB-SubCell.
 GO:0004871; F:signal transducer activity; TAS:ProtInc.
 GO:0003714; F:transcription corepressor activity; TAS:ProtInc.
 GO:0000398; P:mRNA splicing, via spliceosome; IC:UniProtKB. 
Interpro
 IPR020472; G-protein_beta_WD-40_rep.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00320; GPROTEINBRPT.