CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006960
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 1,3-beta-glucan synthase component FKS1 
Protein Synonyms/Alias
 1,3-beta-D-glucan-UDP glucosyltransferase; Calcineurin dependent protein 1; Calcofluor white hypersensitivity protein 53; Echinocandin target gene protein 1; FK506 sensitivity protein 1; Glucan synthase of cerevisiae protein 1; Papulacandin B resistance protein 1 
Gene Name
 FKS1 
Gene Synonyms/Alias
 CND1; CWH53; ETG1; GLS1; GSC1; PBR1; YLR342W; L8300.6 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
222GGDTANYKKWYFAAQubiquitination[1]
245FRNMSLGKLSRKARKubiquitination[1]
259KAKKKNKKAMEEANPubiquitination[1, 2]
275DTEETLNKIEGDNSLubiquitination[1, 2]
292ADFRWKAKMNQLSPLubiquitination[1]
330ECLCFIYKCALDYLDubiquitination[1]
386KRERDHNKIVGYDDLubiquitination[2]
413IVLEDGTKLIELPLEubiquitination[1]
437VWDDVFFKTYKETRTubiquitination[1, 2]
787VPSEIEGKRTLRAPTubiquitination[1]
889VEWECFVKDTKILAEubiquitination[1]
910GNENEAEKEDALKSQubiquitination[1, 2, 3, 4, 5]
915AEKEDALKSQIDDLPubiquitination[2]
929PFYCIGFKSAAPEYTubiquitination[1]
965MNYSRAIKLLYRVENubiquitination[1]
1009VSMQRLAKFKPHELEubiquitination[1]
1011MQRLAKFKPHELENAacetylation[6]
1011MQRLAKFKPHELENAubiquitination[1]
1082NPILGDGKSDNQNHAubiquitination[1]
1172LGDVAAGKEQTFGTLubiquitination[1]
1190TLSQIGGKLHYGHPDubiquitination[1]
1215GGVSKAQKGLHLNEDubiquitination[1]
1539RITGFKRKLVGDESEubiquitination[1, 2]
1547LVGDESEKAAGDASRubiquitination[1, 2]
1788RPPIYSLKQTRLRKRubiquitination[1]
1799LRKRMVKKYCSLYFLubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, VillĂ©n J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery.
 Hitchcock AL, Auld K, Gygi SP, Silver PA.
 Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12735-40. [PMID: 14557538]
 [4] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [5] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269]
 [6] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919
Functional Description
 Alternate catalytic subunit of the 1,3-beta-glucan synthase (GS). Synthesizes 1,3-beta-glucan, a major structural component of the yeast cell wall. Involved in cell wall synthesis, maintenance and cell wall remodeling. 
Sequence Annotation
 MOD_RES 269 269 Phosphothreonine.
 MOD_RES 272 272 Phosphothreonine.
 CROSSLNK 910 910 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Cell membrane; Cell shape; Cell wall biogenesis/degradation; Complete proteome; Glycosyltransferase; Isopeptide bond; Membrane; Mitochondrion; Phosphoprotein; Reference proteome; Transferase; Transmembrane; Transmembrane helix; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1876 AA 
Protein Sequence
MNTDQQPYQG QTDYTQGPGN GQSQEQDYDQ YGQPLYPSQA DGYYDPNVAA GTEADMYGQQ 60
PPNESYDQDY TNGEYYGQPP NMAAQDGENF SDFSSYGPPG TPGYDSYGGQ YTASQMSYGE 120
PNSSGTSTPI YGNYDPNAIA MALPNEPYPA WTADSQSPVS IEQIEDIFID LTNRLGFQRD 180
SMRNMFDHFM VLLDSRSSRM SPDQALLSLH ADYIGGDTAN YKKWYFAAQL DMDDEIGFRN 240
MSLGKLSRKA RKAKKKNKKA MEEANPEDTE ETLNKIEGDN SLEAADFRWK AKMNQLSPLE 300
RVRHIALYLL CWGEANQVRF TAECLCFIYK CALDYLDSPL CQQRQEPMPE GDFLNRVITP 360
IYHFIRNQVY EIVDGRFVKR ERDHNKIVGY DDLNQLFWYP EGIAKIVLED GTKLIELPLE 420
ERYLRLGDVV WDDVFFKTYK ETRTWLHLVT NFNRIWVMHI SIFWMYFAYN SPTFYTHNYQ 480
QLVDNQPLAA YKWASCALGG TVASLIQIVA TLCEWSFVPR KWAGAQHLSR RFWFLCIIFG 540
INLGPIIFVF AYDKDTVYST AAHVVAAVMF FVAVATIIFF SIMPLGGLFT SYMKKSTRRY 600
VASQTFTAAF APLHGLDRWM SYLVWVTVFA AKYSESYYFL VLSLRDPIRI LSTTAMRCTG 660
EYWWGAVLCK VQPKIVLGLV IATDFILFFL DTYLWYIIVN TIFSVGKSFY LGISILTPWR 720
NIFTRLPKRI YSKILATTDM EIKYKPKVLI SQVWNAIIIS MYREHLLAID HVQKLLYHQV 780
PSEIEGKRTL RAPTFFVSQD DNNFETEFFP RDSEAERRIS FFAQSLSTPI PEPLPVDNMP 840
TFTVLTPHYA ERILLSLREI IREDDQFSRV TLLEYLKQLH PVEWECFVKD TKILAEETAA 900
YEGNENEAEK EDALKSQIDD LPFYCIGFKS AAPEYTLRTR IWASLRSQTL YRTISGFMNY 960
SRAIKLLYRV ENPEIVQMFG GNAEGLEREL EKMARRKFKF LVSMQRLAKF KPHELENAEF 1020
LLRAYPDLQI AYLDEEPPLT EGEEPRIYSA LIDGHCEILD NGRRRPKFRV QLSGNPILGD 1080
GKSDNQNHAL IFYRGEYIQL IDANQDNYLE ECLKIRSVLA EFEELNVEQV NPYAPGLRYE 1140
EQTTNHPVAI VGAREYIFSE NSGVLGDVAA GKEQTFGTLF ARTLSQIGGK LHYGHPDFIN 1200
ATFMTTRGGV SKAQKGLHLN EDIYAGMNAM LRGGRIKHCE YYQCGKGRDL GFGTILNFTT 1260
KIGAGMGEQM LSREYYYLGT QLPVDRFLTF YYAHPGFHLN NLFIQLSLQM FMLTLVNLSS 1320
LAHESIMCIY DRNKPKTDVL VPIGCYNFQP AVDWVRRYTL SIFIVFWIAF VPIVVQELIE 1380
RGLWKATQRF FCHLLSLSPM FEVFAGQIYS SALLSDLAIG GARYISTGRG FATSRIPFSI 1440
LYSRFAGSAI YMGARSMLML LFGTVAHWQA PLLWFWASLS SLIFAPFVFN PHQFAWEDFF 1500
LDYRDYIRWL SRGNNQYHRN SWIGYVRMSR ARITGFKRKL VGDESEKAAG DASRAHRTNL 1560
IMAEIIPCAI YAAGCFIAFT FINAQTGVKT TDDDRVNSVL RIIICTLAPI AVNLGVLFFC 1620
MGMSCCSGPL FGMCCKKTGS VMAGIAHGVA VIVHIAFFIV MWVLESFNFV RMLIGVVTCI 1680
QCQRLIFHCM TALMLTREFK NDHANTAFWT GKWYGKGMGY MAWTQPSREL TAKVIELSEF 1740
AADFVLGHVI LICQLPLIII PKIDKFHSIM LFWLKPSRQI RPPIYSLKQT RLRKRMVKKY 1800
CSLYFLVLAI FAGCIIGPAV ASAKIHKHIG DSLDGVVHNL FQPINTTNND TGSQMSTYQS 1860
HYYTHTPSLK TWSTIK 1876 
Gene Ontology
 GO:0000148; C:1,3-beta-D-glucan synthase complex; IDA:SGD.
 GO:0030479; C:actin cortical patch; IDA:SGD.
 GO:0005935; C:cellular bud neck; IDA:SGD.
 GO:0005934; C:cellular bud tip; IDA:SGD.
 GO:0016021; C:integral to membrane; ISM:SGD.
 GO:0005739; C:mitochondrion; IDA:SGD.
 GO:0003843; F:1,3-beta-D-glucan synthase activity; IDA:SGD.
 GO:0006075; P:(1->3)-beta-D-glucan biosynthetic process; IMP:SGD.
 GO:0045807; P:positive regulation of endocytosis; IMP:SGD.
 GO:0008360; P:regulation of cell shape; IEA:UniProtKB-KW.
 GO:0008361; P:regulation of cell size; IMP:SGD. 
Interpro
 IPR026899; FKS1-like_dom1.
 IPR003440; Glyco_trans_48. 
Pfam
 PF14288; FKS1_dom1
 PF02364; Glucan_synthase 
SMART
  
PROSITE
  
PRINTS