CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002701
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Keratin, type II cytoskeletal 7 
Protein Synonyms/Alias
 Cytokeratin-7; CK-7; Keratin-7; K7; Sarcolectin; Type-II keratin Kb7 
Gene Name
 KRT7 
Gene Synonyms/Alias
 SCL 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
96QEESEQIKTLNNKFAubiquitination[1, 2, 3]
117RFLEQQNKLLETKWTubiquitination[1, 2, 3]
122QNKLLETKWTLLQEQubiquitination[1, 2, 3]
130WTLLQEQKSAKSSRLubiquitination[1, 3]
177QDVVEDFKNKYEDEIubiquitination[3]
198ENEFVVLKKDVDAAYubiquitination[3]
199NEFVVLKKDVDAAYMacetylation[4]
214SKVELEAKVDALNDEubiquitination[1, 2, 3]
265DGIIAEVKAQYEEMAubiquitination[2, 3]
273AQYEEMAKCSRAEAEubiquitination[1, 2, 3]
286AEAWYQTKFETLQAQubiquitination[1, 2, 3]
296TLQAQAGKHGDDLRNacetylation[4, 5]
296TLQAQAGKHGDDLRNubiquitination[1, 2, 3]
326QAEIDNIKNQRAKLEubiquitination[1, 2, 3]
331NIKNQRAKLEAAIAEubiquitination[1, 3]
348ERGELALKDARAKQEubiquitination[1, 2, 3]
353ALKDARAKQEELEAAubiquitination[3]
Reference
 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [5] Monoclonal antibody cocktail as an enrichment tool for acetylome analysis.
 Shaw PG, Chaerkady R, Zhang Z, Davidson NE, Pandey A.
 Anal Chem. 2011 May 15;83(10):3623-6. [PMID: 21466224] 
Functional Description
 Blocks interferon-dependent interphase and stimulates DNA synthesis in cells. Involved in the translational regulation of the human papillomavirus type 16 E7 mRNA (HPV16 E7). 
Sequence Annotation
 REGION 2 90 Head.
 REGION 90 126 Coil 1A.
 REGION 91 399 Rod.
 REGION 92 97 Interaction with HPV16 E7.
 REGION 127 144 Linker 1.
 REGION 145 236 Coil 1B.
 REGION 237 260 Linker 12.
 REGION 261 399 Coil 2.
 REGION 400 469 Tail.
 MOD_RES 2 2 N-acetylserine.
 MOD_RES 2 2 Phosphoserine.
 MOD_RES 20 20 Dimethylated arginine; alternate.
 MOD_RES 20 20 Omega-N-methylarginine; alternate.
 MOD_RES 179 179 N6-acetyllysine.
 MOD_RES 252 252 Phosphoserine.
 MOD_RES 254 254 Phosphoserine.  
Keyword
 Acetylation; Coiled coil; Complete proteome; Cytoplasm; Direct protein sequencing; Host-virus interaction; Intermediate filament; Keratin; Methylation; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 469 AA 
Protein Sequence
MSIHFSSPVF TSRSAAFSGR GAQVRLSSAR PGGLGSSSLY GLGASRPRVA VRSAYGGPVG 60
AGIREVTINQ SLLAPLRLDA DPSLQRVRQE ESEQIKTLNN KFASFIDKVR FLEQQNKLLE 120
TKWTLLQEQK SAKSSRLPDI FEAQIAGLRG QLEALQVDGG RLEAELRSMQ DVVEDFKNKY 180
EDEINHRTAA ENEFVVLKKD VDAAYMSKVE LEAKVDALND EINFLRTLNE TELTELQSQI 240
SDTSVVLSMD NSRSLDLDGI IAEVKAQYEE MAKCSRAEAE AWYQTKFETL QAQAGKHGDD 300
LRNTRNEISE MNRAIQRLQA EIDNIKNQRA KLEAAIAEAE ERGELALKDA RAKQEELEAA 360
LQRGKQDMAR QLREYQELMS VKLALDIEIA TYRKLLEGEE SRLAGDGVGA VNISVMNSTG 420
GSSSGGGIGL TLGGTMGSNA LSFSSSAGPG LLKAYSIRTA SASRRSARD 469 
Gene Ontology
 GO:0005794; C:Golgi apparatus; IDA:HPA.
 GO:0005882; C:intermediate filament; NAS:UniProtKB.
 GO:0045095; C:keratin filament; IEA:InterPro.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005198; F:structural molecule activity; NAS:UniProtKB.
 GO:0007010; P:cytoskeleton organization; NAS:UniProtKB.
 GO:0006417; P:regulation of translation; IDA:UniProtKB.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 
Interpro
 IPR016044; F.
 IPR001664; IF.
 IPR018039; Intermediate_filament_CS.
 IPR003054; Keratin_II.
 IPR009053; Prefoldin. 
Pfam
 PF00038; Filament 
SMART
  
PROSITE
 PS00226; IF 
PRINTS
 PR01276; TYPE2KERATIN.