UniProt Accession

 KCC2B_RAT; P08413 

Genbank Protein ID

 M16112 

Genbank Nucleotide ID

 AAA41866.1 

Protein Name

 Calcium/calmodulin-dependent protein kinase type II subunit beta 

Protein Synonyms/Alias

 CaM kinase II subunit beta; CaMK-II subunit beta 

Gene Name

 Camk2b 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
221	FWDEDQHKLYQQIKA	methylation	[1]

Reference

 [1] A method for the comprehensive proteomic analysis of membrane proteins.
 Wu CC, MacCoss MJ, Howell KE, Yates JR 3rd.
 Nat Biotechnol. 2003 May;21(5):532-8. [PMID: 12692561] 

Functional Description

 Calcium/calmodulin-dependent protein kinase that functions autonomously after Ca(2+)/calmodulin-binding and autophosphorylation, and is involved in dendritic spine and synapse formation, neuronal plasticity and regulation of sarcoplasmic reticulum Ca(2+) transport in skeletal muscle. In neurons, plays an essential structural role in the reorganization of the actin cytoskeleton during plasticity by binding and bundling actin filaments in a kinase-independent manner. This structural function is required for correct targeting of CaMK2A, which acts downstream of NMDAR to promote dendritic spine and synapse formation and maintain synaptic plasticity which enables long-term potentiation (LTP) and hippocampus-dependent learning. In developing hippocampal neurons, promotes arborization of the dendritic tree and in mature neurons, promotes dendritic remodeling. Participates in the modulation of skeletal muscle function in response to exercise. In slow-twitch muscles, is involved in regulation of sarcoplasmic reticulum (SR) Ca(2+) transport and in fast-twitch muscle participates in the control of Ca(2+) release from the SR through phosphorylation of triadin, a ryanodine receptor-coupling factor, and phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2. 

Sequence Annotation

 DOMAIN 14 272 Protein kinase.
 NP_BIND 20 28 ATP (By similarity).
 REGION 283 292 Autoinhibitory domain (By similarity).
 REGION 291 301 Calmodulin-binding.
 ACT_SITE 136 136 Proton acceptor (By similarity).
 BINDING 43 43 ATP (By similarity).
 MOD_RES 17 17 Phosphotyrosine (By similarity).
 MOD_RES 287 287 Phosphothreonine; by autocatalysis.
 MOD_RES 306 306 Phosphothreonine; by autocatalysis (By
 MOD_RES 307 307 Phosphothreonine; by autocatalysis (By
 MOD_RES 315 315 Phosphoserine (By similarity).
 MOD_RES 367 367 Phosphoserine (By similarity).
 MOD_RES 397 397 Phosphoserine (By similarity).  

Keyword

 ATP-binding; Calmodulin-binding; Complete proteome; Cytoplasm; Cytoskeleton; Differentiation; Kinase; Membrane; Neurogenesis; Nucleotide-binding; Phosphoprotein; Reference proteome; Sarcoplasmic reticulum; Serine/threonine-protein kinase; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 542 AA 

Protein Sequence

Gene Ontology

 GO:0005954; C:calcium- and calmodulin-dependent protein kinase complex; TAS:UniProtKB.
 GO:0005815; C:microtubule organizing center; IEA:UniProtKB-SubCell.
 GO:0033017; C:sarcoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004683; F:calmodulin-dependent protein kinase activity; IEA:EC.
 GO:0060291; P:long-term synaptic potentiation; IMP:MGI.
 GO:0061003; P:positive regulation of dendritic spine morphogenesis; IMP:UniProtKB.
 GO:0010976; P:positive regulation of neuron projection development; IMP:UniProtKB.
 GO:0090129; P:positive regulation of synapse maturation; IMP:UniProtKB.
 GO:0046777; P:protein autophosphorylation; ISS:UniProtKB. 

Interpro

 IPR020636; Ca/CaM-dep_Ca-dep_prot_Kinase.
 IPR013543; Ca/CaM-dep_prot_kinase-assoc.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF08332; CaMKII_AD
 PF00069; Pkinase 

SMART

 SM00220; S_TKc 

PROSITE

 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS