UniProt Accession

 NECD_HUMAN; Q99608; B2R6Z5 

Genbank Protein ID

 U35139; AB007828; AK312779; BC008750 

Genbank Nucleotide ID

 AAB39469.1; BAA22660.1; BAG35642.1; AAH08750.1 

Protein Name

 Necdin 

Protein Synonyms/Alias

  

Gene Name

 NDN 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
223	ILGLRPWKKHSTFGD	ubiquitination	[1, 2, 3, 4, 5]
272	RASREITKMQIMEFL	ubiquitination	[3, 4]
285	FLARVFKKDPQAWPS	ubiquitination	[3]

Reference

 [1] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 

Functional Description

 Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle- promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences (By similarity). 

Sequence Annotation

 DOMAIN 98 297 MAGE.  

Keyword

 Complete proteome; DNA-binding; Growth regulation; Nucleus; Polymorphism; Reference proteome; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 321 AA 

Protein Sequence

Gene Ontology

 GO:0042995; C:cell projection; IEA:Compara.
 GO:0005813; C:centrosome; IEA:Compara.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0043204; C:perikaryon; IEA:UniProtKB-SubCell.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0007413; P:axonal fasciculation; IEA:Compara.
 GO:0007417; P:central nervous system development; IEA:Compara.
 GO:0071514; P:genetic imprinting; IEA:Compara.
 GO:0008347; P:glial cell migration; IEA:Compara.
 GO:0048871; P:multicellular organismal homeostasis; IEA:Compara.
 GO:0008285; P:negative regulation of cell proliferation; TAS:ProtInc.
 GO:0007399; P:nervous system development; TAS:ProtInc.
 GO:0001764; P:neuron migration; IEA:Compara.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; IEA:Compara.
 GO:0009791; P:post-embryonic development; IEA:Compara.
 GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0003016; P:respiratory system process; IEA:Compara.
 GO:0019233; P:sensory perception of pain; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR002190; MAGE. 

Pfam

 PF01454; MAGE 

SMART

  

PROSITE

 PS50838; MAGE 

PRINTS