CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012028
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Syntaxin-5 
Protein Synonyms/Alias
  
Gene Name
 STX5 
Gene Synonyms/Alias
 STX5A 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
69QEFLSACKSLQTRQNubiquitination[1, 2]
82QNGIQTNKPALRAVRubiquitination[2]
99SEFTLMAKRIGKDLSubiquitination[2]
103LMAKRIGKDLSNTFAubiquitination[2]
114NTFAKLEKLTILAKRubiquitination[2, 3]
128RKSLFDDKAVEIEELubiquitination[2]
148QDINSLNKQIAQLQDubiquitination[2, 4, 5]
Reference
 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 
Functional Description
 Mediates endoplasmic reticulum to Golgi transport (By similarity). 
Sequence Annotation
 DOMAIN 263 325 t-SNARE coiled-coil homology.  
Keyword
 3D-structure; Alternative initiation; Alternative splicing; Coiled coil; Complete proteome; Golgi apparatus; Membrane; Polymorphism; Reference proteome; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 355 AA 
Protein Sequence
MIPRKRYGSK NTDQGVYLGL SKTQVLSPAT AGSSSSDIAP LPPPVTLVPP PPDTMSCRDR 60
TQEFLSACKS LQTRQNGIQT NKPALRAVRQ RSEFTLMAKR IGKDLSNTFA KLEKLTILAK 120
RKSLFDDKAV EIEELTYIIK QDINSLNKQI AQLQDFVRAK GSQSGRHLQT HSNTIVVSLQ 180
SKLASMSNDF KSVLEVRTEN LKQQRSRREQ FSRAPVSALP LAPNHLGGGA VVLGAESHAS 240
KDVAIDMMDS RTSQQLQLID EQDSYIQSRA DTMQNIESTI VELGSIFQQL AHMVKEQEET 300
IQRIDENVLG AQLDVEAAHS EILKYFQSVT SNRWLMVKIF LILIVFFIIF VVFLA 355 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; IEA:Compara.
 GO:0033116; C:endoplasmic reticulum-Golgi intermediate compartment membrane; IEA:UniProtKB-SubCell.
 GO:0005794; C:Golgi apparatus; IDA:HGNC.
 GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0031201; C:SNARE complex; TAS:HGNC.
 GO:0005484; F:SNAP receptor activity; IDA:HGNC.
 GO:0006888; P:ER to Golgi vesicle-mediated transport; IEA:Compara.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0042147; P:retrograde transport, endosome to Golgi; IDA:HGNC.
 GO:0048280; P:vesicle fusion with Golgi apparatus; IEA:Compara.
 GO:0006903; P:vesicle targeting; TAS:ProtInc. 
Interpro
 IPR006012; Syntaxin/epimorphin_CS.
 IPR006011; Syntaxin_N.
 IPR010989; t-SNARE.
 IPR000727; T_SNARE_dom. 
Pfam
 PF05739; SNARE
 PF00804; Syntaxin 
SMART
 SM00397; t_SNARE 
PROSITE
 PS00914; SYNTAXIN
 PS50192; T_SNARE 
PRINTS