CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012522
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Periodic tryptophan protein 2 homolog 
Protein Synonyms/Alias
  
Gene Name
 PWP2 
Gene Synonyms/Alias
 PWP2H 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
43RVTVFDLKNNKSDTLubiquitination[1]
46VFDLKNNKSDTLPLAubiquitination[1, 2]
227PPEGLRLKPPAGWKAubiquitination[2]
233LKPPAGWKADLLQREubiquitination[3, 4]
279VKYSRLAKYFFNKEGubiquitination[2]
284LAKYFFNKEGDFNNLubiquitination[1]
523LASASWDKTVRLWDMubiquitination[1]
662EEFLNRRKMTEFGNLubiquitination[1]
691IPLPGVRKGDMSSRHmethylation[5]
700DMSSRHFKPEIRVTSacetylation[6, 7, 8]
700DMSSRHFKPEIRVTSubiquitination[2]
773ALRLNESKLVQEALEubiquitination[1, 2, 8, 9]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [5] Large-scale global identification of protein lysine methylation in vivo.
 Cao XJ, Arnaudo AM, Garcia BA.
 Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510]
 [6] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [7] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [8] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [9] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931
Functional Description
  
Sequence Annotation
 REPEAT 12 50 WD 1.
 REPEAT 53 93 WD 2.
 REPEAT 94 132 WD 3.
 REPEAT 142 181 WD 4.
 REPEAT 186 225 WD 5.
 REPEAT 286 325 WD 6.
 REPEAT 328 368 WD 7.
 REPEAT 371 410 WD 8.
 REPEAT 413 452 WD 9.
 REPEAT 456 498 WD 10.
 REPEAT 499 538 WD 11.
 REPEAT 541 580 WD 12.
 REPEAT 603 642 WD 13.
 REPEAT 700 740 WD 14.
 MOD_RES 700 700 N6-acetyllysine.
 MOD_RES 746 746 Phosphothreonine.
 MOD_RES 891 891 Phosphoserine.
 MOD_RES 898 898 Phosphoserine.
 MOD_RES 902 902 Phosphoserine.
 MOD_RES 913 913 Phosphoserine.  
Keyword
 Acetylation; Complete proteome; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 919 AA 
Protein Sequence
MKFAYRFSNL LGTVYRRGNL NFTCDGNSVI SPVGNRVTVF DLKNNKSDTL PLATRYNVKC 60
VGLSPDGRLA IIVDEGGDAL LVSLVCRSVL HHFHFKGSVH SVSFSPDGRK FVVTKGNIAQ 120
MYHAPGKKRE FNAFVLDKTY FGPYDETTCI DWTDDSRCFV VGSKDMSTWV FGAERWDNLI 180
YYALGGHKDA IVACFFESNS LDLYSLSQDG VLCMWQCDTP PEGLRLKPPA GWKADLLQRE 240
EEEEEEEDQE GDRETTIRGK ATPAEEEKTG KVKYSRLAKY FFNKEGDFNN LTAAAFHKKS 300
HLLVTGFASG IFHLHELPEF NLIHSLSISD QSIASVAINS SGDWIAFGCS GLGQLLVWEW 360
QSESYVLKQQ GHFNSMVALA YSPDGQYIVT GGDDGKVKVW NTLSGFCFVT FTEHSSGVTG 420
VTFTATGYVV VTSSMDGTVR AFDLHRYRNF RTFTSPRPTQ FSCVAVDASG EIVSAGAQDS 480
FEIFVWSMQT GRLLDVLSGH EGPISGLCFN PMKSVLASAS WDKTVRLWDM FDSWRTKETL 540
ALTSDALAVT FRPDGAELAV ATLNSQITFW DPENAVQTGS IEGRHDLKTG RKELDKITAK 600
HAAKGKAFTA LCYSADGHSI LAGGMSKFVC IYHVREQILM KRFEISCNLS LDAMEEFLNR 660
RKMTEFGNLA LIDQDAGQED GVAIPLPGVR KGDMSSRHFK PEIRVTSLRF SPTGRCWAAT 720
TTEGLLIYSL DTRVLFDPFE LDTSVTPGRV REALRQQDFT RAILMALRLN ESKLVQEALE 780
AVPRGEIEVV TSSLPELYVE KVLEFLASSF EVSRHLEFYL LWTHKLLMLH GQKLKSRAGT 840
LLPVIQFLQK SIQRHLDDLS KLCSWNHYNM QYALAVSKQR GTKRSLDPLG SEEEAEASED 900
DSLHLLGGGG RDSEEEMLA 919 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:HPA.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0004871; F:signal transducer activity; TAS:ProtInc. 
Interpro
 IPR020472; G-protein_beta_WD-40_rep.
 IPR027145; PWP2.
 IPR011047; Quinonprotein_ADH-like_supfam.
 IPR007148; SSU_processome_Utp12.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF04003; Utp12
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00320; GPROTEINBRPT.