CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012476
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Lysine--tRNA ligase 
Protein Synonyms/Alias
 Lysyl-tRNA synthetase; LysRS 
Gene Name
 KARS 
Gene Synonyms/Alias
 KIAA0070 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
106VDPNQYYKIRSQAIHubiquitination[1]
169AKRASGGKLIFYDLRubiquitination[2]
333IAPELYHKMLVVGGIubiquitination[1, 2]
391KMVSGMVKHITGSYKubiquitination[1]
449FETEETRKILDDICVubiquitination[1, 2]
520RFELFVMKKEICNAYubiquitination[1]
Reference
 [1] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [2] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965] 
Functional Description
 Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of diadenosine oligophosphate (Ap4A), a signaling molecule involved in the activation of MITF transcriptional activity. Interacts with HIV-1 virus GAG protein, facilitating the selective packaging of tRNA(3)(Lys), the primer for reverse transcription initiation. 
Sequence Annotation
 NP_BIND 323 325 ATP.
 NP_BIND 331 332 ATP.
 NP_BIND 494 495 ATP.
 NP_BIND 550 553 ATP.
 METAL 487 487 Calcium.
 METAL 494 494 Calcium.
 BINDING 277 277 Substrate; via carbonyl oxygen.
 BINDING 301 301 Substrate.
 BINDING 339 339 Substrate.
 BINDING 341 341 Substrate.
 BINDING 497 497 Substrate.
 BINDING 501 501 Substrate.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 88 88 N6-acetyllysine.
 MOD_RES 141 141 N6-acetyllysine.
 MOD_RES 596 596 Phosphoserine (By similarity).  
Keyword
 3D-structure; Acetylation; Alternative splicing; Aminoacyl-tRNA synthetase; ATP-binding; Cell membrane; Charcot-Marie-Tooth disease; Complete proteome; Cytoplasm; Direct protein sequencing; Disease mutation; Host-virus interaction; Ligase; Membrane; Metal-binding; Mitochondrion; Neuropathy; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Protein biosynthesis; Reference proteome; Secreted. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 597 AA 
Protein Sequence
MLTQAAVRLV RGSLRKTSWA EWGHRELRLG QLAPFTAPHK DKSFSDQRSE LKRRLKAEKK 60
VAEKEAKQKE LSEKQLSQAT AAATNHTTDN GVGPEEESVD PNQYYKIRSQ AIHQLKVNGE 120
DPYPHKFHVD ISLTDFIQKY SHLQPGDHLT DITLKVAGRI HAKRASGGKL IFYDLRGEGV 180
KLQVMANSRN YKSEEEFIHI NNKLRRGDII GVQGNPGKTK KGELSIIPYE ITLLSPCLHM 240
LPHLHFGLKD KETRYRQRYL DLILNDFVRQ KFIIRSKIIT YIRSFLDELG FLEIETPMMN 300
IIPGGAVAKP FITYHNELDM NLYMRIAPEL YHKMLVVGGI DRVYEIGRQF RNEGIDLTHN 360
PEFTTCEFYM AYADYHDLME ITEKMVSGMV KHITGSYKVT YHPDGPEGQA YDVDFTPPFR 420
RINMVEELEK ALGMKLPETN LFETEETRKI LDDICVAKAV ECPPPRTTAR LLDKLVGEFL 480
EVTCINPTFI CDHPQIMSPL AKWHRSKEGL TERFELFVMK KEICNAYTEL NDPMRQRQLF 540
EEQAKAKAAG DDEAMFIDEN FCTALEYGLP PTAGWGMGID RVAMFLTDSN NIKEVLLFPA 600
MKPEDKKENV ATTDTLESTT VGTSV 625 
Gene Ontology
 GO:0017101; C:aminoacyl-tRNA synthetase multienzyme complex; IEA:Compara.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
 GO:0015630; C:microtubule cytoskeleton; IDA:HPA.
 GO:0005759; C:mitochondrial matrix; TAS:Reactome.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0016597; F:amino acid binding; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004824; F:lysine-tRNA ligase activity; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0000049; F:tRNA binding; NAS:UniProtKB.
 GO:0015966; P:diadenosine tetraphosphate biosynthetic process; IEA:Compara.
 GO:0006430; P:lysyl-tRNA aminoacylation; IDA:UniProtKB.
 GO:0008033; P:tRNA processing; NAS:UniProtKB.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 
Interpro
 IPR004364; aa-tRNA-synt_II.
 IPR018150; aa-tRNA-synt_II-like.
 IPR006195; aa-tRNA-synth_II.
 IPR002313; Lys-tRNA-ligase_II.
 IPR018149; Lys-tRNA-synth_II_C.
 IPR012340; NA-bd_OB-fold.
 IPR004365; NA-bd_OB_tRNA-helicase. 
Pfam
 PF00152; tRNA-synt_2
 PF01336; tRNA_anti 
SMART
  
PROSITE
 PS50862; AA_TRNA_LIGASE_II 
PRINTS
 PR00982; TRNASYNTHLYS.