CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-007528
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mannose-1-phosphate guanyltransferase 
Protein Synonyms/Alias
 ATP-mannose-1-phosphate guanylyltransferase; GDP-mannose pyrophosphorylase; NDP-hexose pyrophosphorylase 
Gene Name
 PSA1 
Gene Synonyms/Alias
 MPG1; SRB1; VIG9; YDL055C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
98KLAEDVLKKDNSPFFacetylation[1]
125KELADFHKAHGGKGTacetylation[1]
130FHKAHGGKGTIVATKubiquitination[2]
137KGTIVATKVDEPSKYacetylation[1]
137KGTIVATKVDEPSKYubiquitination[2]
143TKVDEPSKYGVIVHDacetylation[1]
143TKVDEPSKYGVIVHDubiquitination[2]
196MKPTSIEKETFPILVubiquitination[2]
239LYLNSLAKRQPKKLAubiquitination[2]
244LAKRQPKKLATGANIubiquitination[2, 3]
268AKISSTAKIGPDVVIacetylation[1]
268AKISSTAKIGPDVVIubiquitination[2]
299VLCNSTIKNHSLVKSacetylation[1]
299VLCNSTIKNHSLVKSmethylation[4]
299VLCNSTIKNHSLVKSubiquitination[2]
305IKNHSLVKSTIVGWNubiquitination[2]
348GGKVLPHKSISDNVPacetylation[1]
348GGKVLPHKSISDNVPubiquitination[2, 3]
356SISDNVPKEAIIM**acetylation[1]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, VillĂ©n J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [4] Identification of arginine- and lysine-methylation in the proteome of Saccharomyces cerevisiae and its functional implications.
 Pang CN, Gasteiger E, Wilkins MR.
 BMC Genomics. 2010 Feb 5;11:92. [PMID: 20137074
Functional Description
 Involved in cell wall synthesis where it is required for glycosylation. Involved in cell cycle progression through cell- size checkpoint. 
Sequence Annotation
 MOD_RES 153 153 Phosphothreonine.  
Keyword
 Cell cycle; Complete proteome; Cytoplasm; GTP-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 361 AA 
Protein Sequence
MKGLILVGGY GTRLRPLTLT VPKPLVEFGN RPMILHQIEA LANAGVTDIV LAVNYRPEVM 60
VETLKKYEKE YGVNITFSVE TEPLGTAGPL KLAEDVLKKD NSPFFVLNSD VICEYPFKEL 120
ADFHKAHGGK GTIVATKVDE PSKYGVIVHD IATPNLIDRF VEKPKEFVGN RINAGLYILN 180
PEVIDLIEMK PTSIEKETFP ILVEEKQLYS FDLEGFWMDV GQPKDFLSGT VLYLNSLAKR 240
QPKKLATGAN IVGNALIDPT AKISSTAKIG PDVVIGPNVT IGDGVRITRS VVLCNSTIKN 300
HSLVKSTIVG WNSTVGQWCR LEGVTVLGDD VEVKDEIYIN GGKVLPHKSI SDNVPKEAII 360
M 361 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
 GO:0004475; F:mannose-1-phosphate guanylyltransferase activity; IDA:SGD.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0000032; P:cell wall mannoprotein biosynthetic process; IMP:SGD.
 GO:0009298; P:GDP-mannose biosynthetic process; IDA:SGD.
 GO:0006486; P:protein glycosylation; IMP:SGD. 
Interpro
 IPR001451; Hexapep_transf.
 IPR018357; Hexapep_transf_CS.
 IPR005835; NTP_transferase. 
Pfam
 PF00132; Hexapep
 PF00483; NTP_transferase 
SMART
  
PROSITE
 PS00101; HEXAPEP_TRANSFERASES 
PRINTS