UniProt Accession

 EIF3F_MOUSE; Q9DCH4; Q5XJV3 

Genbank Protein ID

 AK002778; CH466531; BC083190 

Genbank Nucleotide ID

 BAB22352.1; EDL16920.1; AAH83190.1 

Protein Name

 Eukaryotic translation initiation factor 3 subunit F 

Protein Synonyms/Alias

 eIF3f; Deubiquitinating enzyme eIF3f; Eukaryotic translation initiation factor 3 subunit 5; eIF-3-epsilon; eIF3 p47 

Gene Name

 Eif3f 

Gene Synonyms/Alias

 Eif3s5 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
242	MFTPLTVKYAYYDTE	acetylation	[1]
242	MFTPLTVKYAYYDTE	ubiquitination	[2]

Reference

 [1] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [2] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. 

Sequence Annotation

 DOMAIN 91 200 MPN.
 MOD_RES 2 2 N-acetylalanine (By similarity).
 MOD_RES 52 52 Phosphoserine; by CDK11; in vitro (By
 MOD_RES 242 242 N6-acetyllysine (By similarity).
 MOD_RES 262 262 Phosphoserine (By similarity).  

Keyword

 Acetylation; Complete proteome; Cytoplasm; Hydrolase; Initiation factor; Phosphoprotein; Protease; Protein biosynthesis; Reference proteome; Thiol protease; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 361 AA 

Protein Sequence

Gene Ontology

 GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:HAMAP.
 GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:HAMAP.
 GO:0005852; C:eukaryotic translation initiation factor 3 complex; IDA:UniProtKB.
 GO:0008234; F:cysteine-type peptidase activity; IEA:UniProtKB-KW.
 GO:0003743; F:translation initiation factor activity; IEA:HAMAP.
 GO:0001731; P:formation of translation preinitiation complex; IEA:HAMAP.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
 GO:0006446; P:regulation of translational initiation; IEA:HAMAP.
 GO:0006413; P:translational initiation; IDA:UniProtKB. 

Interpro

 IPR027531; eIF3f.
 IPR000555; JAB1_Mov34_MPN_PAD1.
 IPR024969; Rpn11/EIF3F_C. 

Pfam

 PF01398; JAB
 PF13012; MitMem_reg 

SMART

 SM00232; JAB_MPN 

PROSITE

  

PRINTS