CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012389
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Chromobox protein homolog 2 
Protein Synonyms/Alias
  
Gene Name
 CBX2 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
61LLLAFQKKEHEKEVQubiquitination[1]
96CSRRSKLKEPDAPSKubiquitination[1]
215VAGLAALKAHAKEACubiquitination[1, 2]
277AASRLALKAQATNKCubiquitination[1]
283LKAQATNKCGLGLDLubiquitination[1, 2]
291CGLGLDLKVRTQKGEubiquitination[1]
390ATNPAPGKGTGSGLIubiquitination[1]
427APPTPASKRDCVKGSubiquitination[1]
432ASKRDCVKGSATPSGubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Component of a Polycomb group (PcG) multiprotein PRC1- like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Involved in sexual development, acting as activator of NR5A1 expression. 
Sequence Annotation
 DOMAIN 12 70 Chromo.
 DNA_BIND 75 87 A.T hook.
 MOTIF 163 168 Nuclear localization signal (Potential).  
Keyword
 3D-structure; Alternative splicing; Chromatin regulator; Complete proteome; Differentiation; Disease mutation; DNA-binding; Nucleus; Reference proteome; Repressor; Sexual differentiation; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 532 AA 
Protein Sequence
MEELSSVGEQ VFAAECILSK RLRKGKLEYL VKWRGWSSKH NSWEPEENIL DPRLLLAFQK 60
KEHEKEVQNR KRGKRPRGRP RKLTAMSSCS RRSKLKEPDA PSKSKSSSSS SSSTSSSSSS 120
DEEDDSDLDA KRGPRGRETH PVPQKKAQIL VAKPELKDPI RKKRGRKPLP PEQKATRRPV 180
SLAKVLKTAR KDLGAPASKL PPPLSAPVAG LAALKAHAKE ACGGPSAMAT PENLASLMKG 240
MASSPGRGGI SWQSSIVHYM NRMTQSQAQA ASRLALKAQA TNKCGLGLDL KVRTQKGELG 300
MSPPGSKIPK APSGGAVEQK VGNTGGPPHT HGASRVPAGC PGPQPAPTQE LSLQVLDLQS 360
VKNGMPGVGL LARHATATKG VPATNPAPGK GTGSGLIGAS GATMPTDTSK SEKLASRAVA 420
PPTPASKRDC VKGSATPSGQ ESRTAPGEAR KAATLPEMSA GEESSSSDSD PDSASPPSTG 480
QNPSVSVQTS QDWKPTRSLI EHVFVTDVTA NLITVTVKES PTSVGFFNLR HY 532 
Gene Ontology
 GO:0000792; C:heterochromatin; IEA:Compara.
 GO:0035102; C:PRC1 complex; IDA:UniProtKB.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
 GO:0016568; P:chromatin modification; NAS:UniProtKB.
 GO:0045137; P:development of primary sexual characteristics; IMP:UniProtKB.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IMP:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR017956; AT_hook_DNA-bd_motif.
 IPR023780; Chromo_domain.
 IPR000953; Chromo_domain/shadow.
 IPR016197; Chromodomain-like.
 IPR023779; Chromodomain_CS. 
Pfam
 PF00385; Chromo 
SMART
 SM00384; AT_hook
 SM00298; CHROMO 
PROSITE
 PS00598; CHROMO_1
 PS50013; CHROMO_2 
PRINTS