UniProt Accession

 BUB1_HUMAN; O43683; O43430; O43643; O60626; Q53QE4 

Genbank Protein ID

 AF046078; AF043294; AF053305; AF047471; AF139363; AF139349; AF139350; AF139351; AF139352; AF139353; AF139354; AF139355; AF139356; AF139357; AF139358; AF139359; AF139360; AF139361; AF139362; AC114776; CH471237; BC028201; AF011387 

Genbank Nucleotide ID

 AAC12729.1; AAB97855.2; AAC06259.1; AAC03122.1; AAD43675.1; AAD43675.1; AAD43675.1; AAD43675.1; AAD43675.1; AAD43675.1; AAD43675.1; AAD43675.1; AAD43675.1; AAD43675.1; AAD43675.1; AAD43675.1; AAD43675.1; AAD43675.1; AAD43675.1; AAY14706.1; EAW50355.1; AAH28201.1; AAC39546.1 

Protein Name

 Mitotic checkpoint serine/threonine-protein kinase BUB1 

Protein Synonyms/Alias

 hBUB1; BUB1A 

Gene Name

 BUB1 

Gene Synonyms/Alias

 BUB1L 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
175	LNQMITSKSNPGNNM	ubiquitination	[1, 2]
187	NNMACISKNQGSELS	ubiquitination	[2]
203	VISSACDKESNMERR	ubiquitination	[2]
216	RRVITISKSEYSVHS	ubiquitination	[2]
241	VVMYCKEKLIRGESE	ubiquitination	[2]
264	QKYNQRRKHEQWVNE	ubiquitination	[2]
279	DRHYMKRKEANAFEE	ubiquitination	[2]
290	AFEEQLLKQKMDELH	ubiquitination	[2]
299	KMDELHKKLHQVVET	ubiquitination	[1, 2]
353	QTPVNMEKNPREAPP	ubiquitination	[2]
403	SMFAVASKDAGCVNK	ubiquitination	[2]
455	MVQATPSKVQPSPTV	ubiquitination	[2]
535	HVFEDGNKENYGLPQ	ubiquitination	[2]
564	SVSRLPSKPKEEVPH	ubiquitination	[2]
566	SRLPSKPKEEVPHAE	ubiquitination	[2]
588	VWGIRCNKTLAPSPK	ubiquitination	[2]
653	VVPSRDGKFSPIQEK	ubiquitination	[2]
660	KFSPIQEKSPKQALS	ubiquitination	[2]
663	PIQEKSPKQALSSHM	ubiquitination	[2]
756	KLLSGLSKPVSSYPN	ubiquitination	[2]
777	KLPAIKPKTEFQLGS	ubiquitination	[2]
815	DLNDAKNKQKFVLKV	ubiquitination	[2]
1056	LLRQKLKKVFQQHYT	ubiquitination	[2]
1081	IVLLLECKRSRK***	ubiquitination	[2]

Reference

 [1] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 Serine/threonine-protein kinase that performs 2 crucial functions during mitosis: it is essential for spindle-assembly checkpoint signaling and for correct chromosome alignment. Has a key role in the assembly of checkpoint proteins at the kinetochore, being required for the subsequent localization of CENPF, BUB1B, CENPE and MAD2L1. Required for the kinetochore localization of PLK1. Plays an important role in defining SGOL1 localization and thereby affects sister chromatid cohesion. Acts as a substrate for anaphase-promoting complex or cyclosome (APC/C) in complex with its activator CDH1 (APC/C-Cdh1). Necessary for ensuring proper chromosome segregation and binding to BUB3 is essential for this function. Can regulate chromosome segregation in a kinetochore-independent manner. Can phosphorylate BUB3. The BUB1-BUB3 complex plays a role in the inhibition of APC/C when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1. Kinase activity is essential for inhibition of APC/CCDC20 and for chromosome alignment but does not play a major role in the spindle-assembly checkpoint activity. Mediates cell death in response to chromosome missegregation and acts to suppress spontaneous tumorigenesis. 

Sequence Annotation

 DOMAIN 11 182 BUB1 N-terminal.
 DOMAIN 787 1085 Protein kinase.
 NP_BIND 793 801 ATP (By similarity).
 REGION 1 146 Necessary for kinetochore localization.
 REGION 99 132 Necessary for interaction with CASC5.
 REGION 229 256 Necessary for interaction with BUB3.
 REGION 458 476 Essential for loading of BUBR1, MAD1L1
 MOTIF 58 65 Nuclear localization signal (Potential).
 MOTIF 535 537 KEN box 1.
 MOTIF 625 627 KEN box 2.
 ACT_SITE 917 917 Proton acceptor (By similarity).
 BINDING 821 821 ATP (By similarity).
 MOD_RES 314 314 Phosphoserine.
 MOD_RES 375 375 Phosphoserine.
 MOD_RES 563 563 Phosphoserine.
 MOD_RES 593 593 Phosphoserine.
 MOD_RES 596 596 Phosphoserine.
 MOD_RES 609 609 Phosphothreonine; by CDK1.
 MOD_RES 655 655 Phosphoserine.  

Keyword

 3D-structure; Apoptosis; ATP-binding; Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition; Complete proteome; Host-virus interaction; Kinase; Kinetochore; Mitosis; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Serine/threonine-protein kinase; Transferase; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 1085 AA 

Protein Sequence

Gene Ontology

 GO:0000777; C:condensed chromosome kinetochore; IDA:UniProtKB.
 GO:0000780; C:condensed nuclear chromosome, centromeric region; IEA:Compara.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004672; F:protein kinase activity; TAS:ProtInc.
 GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
 GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0008283; P:cell proliferation; IEA:Compara.
 GO:0007059; P:chromosome segregation; IEA:UniProtKB-KW.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome.
 GO:0007094; P:mitotic spindle assembly checkpoint; TAS:ProtInc.
 GO:0007063; P:regulation of sister chromatid cohesion; IDA:UniProtKB.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 

Interpro

 IPR015661; Bub1/Mad3.
 IPR011009; Kinase-like_dom.
 IPR013212; Mad3_BUB1_I.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR008271; Ser/Thr_kinase_AS. 

Pfam

 PF08311; Mad3_BUB1_I
 PF00069; Pkinase 

SMART

 SM00777; Mad3_BUB1_I 

PROSITE

 PS51489; BUB1_N
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 

PRINTS