UniProt Accession

 MCES_MOUSE; Q9D0L8; Q3V3U9; Q6ZQC6; Q9D5F1 

Genbank Protein ID

 AK129130; AK011300; AK015403; AK031780; BC021794; AK082331 

Genbank Nucleotide ID

 BAC97940.1; BAB27527.1; BAB29834.1; BAE43278.1; AAH21794.1; BAC38469.1 

Protein Name

 mRNA cap guanine-N7 methyltransferase 

Protein Synonyms/Alias

 RG7MT1; mRNA (guanine-N(7)-)-methyltransferase; mRNA cap methyltransferase 

Gene Name

 Rnmt 

Gene Synonyms/Alias

 Kiaa0398 

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
197	VLDLGCGKGGDLLKW	ubiquitination	[1]
398	KIKNNENKMLLKRMQ	acetylation	[2]
402	NENKMLLKRMQALEQ	acetylation	[2]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753] 

Functional Description

 mRNA-capping methyltransferase that methylates the N7 position of the added guanosine to the 5'-cap structure of mRNAs. Binds RNA containing 5'-terminal GpppC (By similarity). 

Sequence Annotation

 DOMAIN 117 465 mRNA cap 0 methyltransferase.
 REGION 165 166 mRNA cap binding (By similarity).
 REGION 250 251 S-adenosyl-L-methionine binding (By
 REGION 273 275 S-adenosyl-L-methionine binding (By
 MOTIF 113 115 Nuclear localization signal (By
 BINDING 169 169 S-adenosyl-L-methionine (By similarity).
 BINDING 194 194 S-adenosyl-L-methionine; via carbonyl
 BINDING 216 216 S-adenosyl-L-methionine (By similarity).
 BINDING 277 277 mRNA cap (By similarity).
 BINDING 359 359 mRNA cap (By similarity).
 BINDING 456 456 mRNA cap (By similarity).
 MOD_RES 11 11 Phosphoserine.
 MOD_RES 15 15 Phosphoserine.
 MOD_RES 64 64 Phosphoserine.
 MOD_RES 100 100 Phosphoserine.  

Keyword

 Alternative splicing; Complete proteome; Methyltransferase; mRNA capping; mRNA processing; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; S-adenosyl-L-methionine; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 465 AA 

Protein Sequence

Gene Ontology

 GO:0005845; C:mRNA cap binding complex; ISS:UniProtKB.
 GO:0005730; C:nucleolus; IEA:Compara.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0004482; F:mRNA (guanine-N7-)-methyltransferase activity; ISS:UniProtKB.
 GO:0003723; F:RNA binding; ISS:UniProtKB. 

Interpro

 IPR016899; mRNA_G-N7_MeTrfase.
 IPR004971; mRNA_G-N7_MeTrfase_dom. 

Pfam

 PF03291; Pox_MCEL 

SMART

  

PROSITE

 PS51562; RNA_CAP0_MT 

PRINTS