CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000716
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Krueppel-like factor 4 
Protein Synonyms/Alias
 Epithelial zinc finger protein EZF; Gut-enriched krueppel-like factor 
Gene Name
 KLF4 
Gene Synonyms/Alias
 EZF; GKLF 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
32SGPAGREKTLRQAGAubiquitination[1, 2]
Reference
 [1] Proteomic analysis of ubiquitinated proteins in normal hepatocyte cell line Chang liver cells.
 Tan F, Lu L, Cai Y, Wang J, Xie Y, Wang L, Gong Y, Xu BE, Wu J, Luo Y, Qiang B, Yuan J, Sun X, Peng X.
 Proteomics. 2008 Jul;8(14):2885-96. [PMID: 18655026]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. 
Sequence Annotation
 ZN_FING 430 454 C2H2-type 1.
 ZN_FING 460 484 C2H2-type 2.
 ZN_FING 490 512 C2H2-type 3.
 REGION 473 504 Interaction with target DNA (By
 MOTIF 101 109 9aaTAD.
 MOD_RES 254 254 Phosphoserine.
 CROSSLNK 32 32 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Activator; Alternative splicing; Complete proteome; DNA-binding; Isopeptide bond; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation; Ubl conjugation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 513 AA 
Protein Sequence
MRQPPGESDM AVSDALLPSF STFASGPAGR EKTLRQAGAP NNRWREELSH MKRLPPVLPG 60
RPYDLAAATV ATDLESGGAG AACGGSNLAP LPRRETEEFN DLLDLDFILS NSLTHPPESV 120
AATVSSSASA SSSSSPSSSG PASAPSTCSF TYPIRAGNDP GVAPGGTGGG LLYGRESAPP 180
PTAPFNLADI NDVSPSGGFV AELLRPELDP VYIPPQQPQP PGGGLMGKFV LKASLSAPGS 240
EYGSPSVISV SKGSPDGSHP VVVAPYNGGP PRTCPKIKQE AVSSCTHLGA GPPLSNGHRP 300
AAHDFPLGRQ LPSRTTPTLG LEEVLSSRDC HPALPLPPGF HPHPGPNYPS FLPDQMQPQV 360
PPLHYQGQSR GFVARAGEPC VCWPHFGTHG MMLTPPSSPL ELMPPGSCMP EEPKPKRGRR 420
SWPRKRTATH TCDYAGCGKT YTKSSHLKAH LRTHTGEKPY HCDWDGCGWK FARSDELTRH 480
YRKHTGHRPF QCQKCDRAFS RSDHLALHMK RHF 513 
Gene Ontology
 GO:0000790; C:nuclear chromatin; IDA:BHF-UCL.
 GO:0005667; C:transcription factor complex; IEA:Compara.
 GO:0000987; F:core promoter proximal region sequence-specific DNA binding; IDA:BHF-UCL.
 GO:0003690; F:double-stranded DNA binding; IEA:Compara.
 GO:0035014; F:phosphatidylinositol 3-kinase regulator activity; IEA:Compara.
 GO:0001077; F:RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription; IMP:BHF-UCL.
 GO:0001085; F:RNA polymerase II transcription factor binding; ISS:BHF-UCL.
 GO:0001190; F:RNA polymerase II transcription factor binding transcription factor activity involved in positive regulation of transcription; IDA:BHF-UCL.
 GO:0001010; F:sequence-specific DNA binding transcription factor recruiting transcription factor activity; ISS:BHF-UCL.
 GO:0008270; F:zinc ion binding; NAS:UniProtKB.
 GO:0071363; P:cellular response to growth factor stimulus; IDA:BHF-UCL.
 GO:0009913; P:epidermal cell differentiation; IEA:Compara.
 GO:0048730; P:epidermis morphogenesis; IEA:Compara.
 GO:0045444; P:fat cell differentiation; ISS:BHF-UCL.
 GO:0007500; P:mesodermal cell fate determination; TAS:ProtInc.
 GO:0090051; P:negative regulation of cell migration involved in sprouting angiogenesis; IDA:BHF-UCL.
 GO:0008285; P:negative regulation of cell proliferation; TAS:ProtInc.
 GO:2000342; P:negative regulation of chemokine (C-X-C motif) ligand 2 production; IDA:BHF-UCL.
 GO:0043154; P:negative regulation of cysteine-type endopeptidase activity involved in apoptotic process; IDA:BHF-UCL.
 GO:0070373; P:negative regulation of ERK1 and ERK2 cascade; IEA:Compara.
 GO:0034115; P:negative regulation of heterotypic cell-cell adhesion; IDA:BHF-UCL.
 GO:0050728; P:negative regulation of inflammatory response; TAS:BHF-UCL.
 GO:0045415; P:negative regulation of interleukin-8 biosynthetic process; IDA:BHF-UCL.
 GO:0014740; P:negative regulation of muscle hyperplasia; IEA:Compara.
 GO:0032088; P:negative regulation of NF-kappaB transcription factor activity; IDA:BHF-UCL.
 GO:0014067; P:negative regulation of phosphatidylinositol 3-kinase cascade; IEA:Compara.
 GO:0051898; P:negative regulation of protein kinase B signaling cascade; IEA:Compara.
 GO:0060761; P:negative regulation of response to cytokine stimulus; IDA:BHF-UCL.
 GO:0048662; P:negative regulation of smooth muscle cell proliferation; IEA:Compara.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:BHF-UCL.
 GO:0046985; P:positive regulation of hemoglobin biosynthetic process; IMP:BHF-UCL.
 GO:0031077; P:post-embryonic camera-type eye development; IEA:Compara.
 GO:0035166; P:post-embryonic hemopoiesis; IMP:BHF-UCL.
 GO:0032526; P:response to retinoic acid; IEA:Compara.
 GO:0019827; P:stem cell maintenance; ISS:UniProtKB. 
Interpro
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
  
SMART
 SM00355; ZnF_C2H2 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS