UniProt Accession

 TFAM_HUMAN; Q00059; A8MRB2; A9QXC6; B5BU05; Q5U0C6 

Genbank Protein ID

 M62810; EU279428; BT019658; BT019659; AK312558; AB451241; AB451366; AC023170; BC126366; X64269 

Genbank Nucleotide ID

 AAA59849.1; ABX72056.1; AAV38464.1; AAV38465.1; BAG35455.1; BAG70055.1; BAG70180.1; AAI26367.1; CAA45562.1 

Protein Name

 Transcription factor A, mitochondrial 

Protein Synonyms/Alias

 mtTFA; Mitochondrial transcription factor 1; MtTF1; Transcription factor 6; TCF-6; Transcription factor 6-like 2 

Gene Name

 TFAM 

Gene Synonyms/Alias

 TCF6; TCF6L2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
52	VLASCPKKPVSSYLR	ubiquitination	[1]
62	SSYLRFSKEQLPIFK	ubiquitination	[2]
118	KEEISRFKEQLTPSQ	ubiquitination	[2]

Reference

 [1] Mass spectrometric analysis of lysine ubiquitylation reveals promiscuity at site level.
 Danielsen JM, Sylvestersen KB, Bekker-Jensen S, Szklarczyk D, Poulsen JW, Horn H, Jensen LJ, Mailand N, Nielsen ML.
 Mol Cell Proteomics. 2011 Mar;10(3):M110.003590. [PMID: 21139048]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 

Functional Description

 Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA. 

Sequence Annotation

 DNA_BIND 50 118 HMG box 1.
 DNA_BIND 155 219 HMG box 2.
 MOD_RES 55 55 Phosphoserine; by PKA.
 MOD_RES 56 56 Phosphoserine; by PKA.
 MOD_RES 61 61 Phosphoserine; by PKA.
 MOD_RES 160 160 Phosphoserine; by PKA.
 MOD_RES 193 193 Phosphoserine.  

Keyword

 3D-structure; Activator; Alternative splicing; Complete proteome; Direct protein sequencing; DNA-binding; Mitochondrion; Mitochondrion nucleoid; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transcription; Transcription regulation; Transit peptide. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 246 AA 

Protein Sequence

Gene Ontology

 GO:0042645; C:mitochondrial nucleoid; IDA:BHF-UCL.
 GO:0003682; F:chromatin binding; IDA:UniProtKB.
 GO:0008301; F:DNA binding, bending; IDA:UniProtKB.
 GO:0070363; F:mitochondrial light strand promoter sense binding; IDA:UniProtKB.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IMP:UniProtKB.
 GO:0006261; P:DNA-dependent DNA replication; TAS:ProtInc.
 GO:0033108; P:mitochondrial respiratory chain complex assembly; IEA:Compara.
 GO:0045893; P:positive regulation of transcription, DNA-dependent; IDA:UniProtKB.
 GO:0006356; P:regulation of transcription from RNA polymerase I promoter; TAS:ProtInc.
 GO:0006391; P:transcription initiation from mitochondrial promoter; IDA:UniProtKB. 

Interpro

 IPR009071; HMG_box_dom. 

Pfam

 PF09011; DUF1898
 PF00505; HMG_box 

SMART

 SM00398; HMG 

PROSITE

 PS50118; HMG_BOX_2 

PRINTS