UniProt Accession

 EIF3J_RAT; A0JPM9 

Genbank Protein ID

 BC127509 

Genbank Nucleotide ID

 AAI27510.1 

Protein Name

 Eukaryotic translation initiation factor 3 subunit J 

Protein Synonyms/Alias

 eIF3j; Eukaryotic translation initiation factor 3 subunit 1; eIF-3-alpha; eIF3 p35 

Gene Name

 Eif3j 

Gene Synonyms/Alias

 Eif3s1 

Created Date

 July 27, 2013 

Organism

 Rattus norvegicus (Rat) 

NCBI Taxa ID

 10116 

Lysine Modification

Position	Peptide	Type	References
118	PEEQLADKLRLKKLQ	acetylation	[1]

Reference

 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405] 

Functional Description

 Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. This subunit binds directly within the mRNA entry channel of the 40S ribosome to the aminoacyl (A) site. It may regulate the interaction between the 43S PIC and mRNA (By similarity). 

Sequence Annotation

 REGION 1 70 Sufficient for interaction with EIF3B (By
 REGION 244 259 Promotes stable association with the 40S
 MOD_RES 12 12 Phosphoserine (By similarity).
 MOD_RES 14 14 Phosphoserine (By similarity).
 MOD_RES 21 21 Phosphoserine (By similarity).
 MOD_RES 110 110 Phosphothreonine (By similarity).
 MOD_RES 128 128 Phosphoserine (By similarity).
 MOD_RES 255 255 Phosphotyrosine (By similarity).  

Keyword

 Coiled coil; Complete proteome; Cytoplasm; Initiation factor; Phosphoprotein; Protein biosynthesis; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 259 AA 

Protein Sequence

Gene Ontology

 GO:0016282; C:eukaryotic 43S preinitiation complex; IEA:HAMAP.
 GO:0033290; C:eukaryotic 48S preinitiation complex; IEA:HAMAP.
 GO:0005852; C:eukaryotic translation initiation factor 3 complex; ISS:UniProtKB.
 GO:0003743; F:translation initiation factor activity; IEA:HAMAP.
 GO:0001731; P:formation of translation preinitiation complex; IEA:HAMAP.
 GO:0006446; P:regulation of translational initiation; IEA:HAMAP. 

Interpro

 IPR023194; eIF3-like_dom.
 IPR013906; eIF3j. 

Pfam

 PF08597; eIF3_subunit 

SMART

  

PROSITE

  

PRINTS