CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021654
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 E3 ubiquitin-protein ligase SMURF1 
Protein Synonyms/Alias
 hSMURF1; SMAD ubiquitination regulatory factor 1; SMAD-specific E3 ubiquitin-protein ligase 1 
Gene Name
 SMURF1 
Gene Synonyms/Alias
 KIAA1625 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
350MNHQCQLKEPSQPLPubiquitination[1, 2]
381YERDLVQKLKVLRHEubiquitination[3]
383RDLVQKLKVLRHELSubiquitination[3]
Reference
 [1] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA. 
Sequence Annotation
 DOMAIN 1 99 C2.
 DOMAIN 234 267 WW 1.
 DOMAIN 306 339 WW 2.
 DOMAIN 420 757 HECT.
 ACT_SITE 725 725 Glycyl thioester intermediate.
 CROSSLNK 381 381 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 383 383 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Alternative splicing; Cell membrane; Complete proteome; Cytoplasm; Isopeptide bond; Ligase; Membrane; Polymorphism; Reference proteome; Repeat; Ubl conjugation; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 757 AA 
Protein Sequence
MSNPGTRRNG SSIKIRLTVL CAKNLAKKDF FRLPDPFAKI VVDGSGQCHS TDTVKNTLDP 60
KWNQHYDLYV GKTDSITISV WNHKKIHKKQ GAGFLGCVRL LSNAISRLKD TGYQRLDLCK 120
LNPSDTDAVR GQIVVSLQTR DRIGTGGSVV DCRGLLENEG TVYEDSGPGR PLSCFMEEPA 180
PYTDSTGAAA GGGNCRFVES PSQDQRLQAQ RLRNPDVRGS LQTPQNRPHG HQSPELPEGY 240
EQRTTVQGQV YFLHTQTGVS TWHDPRIPSP SGTIPGGDAA FLYEFLLQGH TSEPRDLNSV 300
NCDELGPLPP GWEVRSTVSG RIYFVDHNNR TTQFTDPRLH HIMNHQCQLK EPSQPLPLPS 360
EGSLEDEELP AQRYERDLVQ KLKVLRHELS LQQPQAGHCR IEVSREEIFE ESYRQIMKMR 420
PKDLKKRLMV KFRGEEGLDY GGVAREWLYL LCHEMLNPYY GLFQYSTDNI YMLQINPDSS 480
INPDHLSYFH FVGRIMGLAV FHGHYINGGF TVPFYKQLLG KPIQLSDLES VDPELHKSLV 540
WILENDITPV LDHTFCVEHN AFGRILQHEL KPNGRNVPVT EENKKEYVRL YVNWRFMRGI 600
EAQFLALQKG FNELIPQHLL KPFDQKELEL IIGGLDKIDL NDWKSNTRLK HCVADSNIVR 660
WFWQAVETFD EERRARLLQF VTGSTRVPLQ GFKALQGSTG AAGPRLFTIH LIDANTDNLP 720
KAHTCFNRID IPPYESYEKL YEKLLTAVEE TCGFAVE 757 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0043025; C:neuronal cell body; IEA:Compara.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005886; C:plasma membrane; IDA:BHF-UCL.
 GO:0004842; F:ubiquitin-protein ligase activity; IDA:UniProtKB.
 GO:0030509; P:BMP signaling pathway; IDA:UniProtKB.
 GO:0030154; P:cell differentiation; IDA:UniProtKB.
 GO:0007398; P:ectoderm development; TAS:UniProtKB.
 GO:0030514; P:negative regulation of BMP signaling pathway; TAS:UniProtKB.
 GO:0030279; P:negative regulation of ossification; IEA:Compara.
 GO:0030512; P:negative regulation of transforming growth factor beta receptor signaling pathway; IDA:BHF-UCL.
 GO:0031398; P:positive regulation of protein ubiquitination; IDA:BHF-UCL.
 GO:0043161; P:proteasomal ubiquitin-dependent protein catabolic process; IDA:BHF-UCL.
 GO:0006611; P:protein export from nucleus; IDA:BHF-UCL.
 GO:0034394; P:protein localization to cell surface; IDA:BHF-UCL.
 GO:0000209; P:protein polyubiquitination; IDA:UniProtKB.
 GO:0042787; P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process; IDA:UniProtKB.
 GO:0032801; P:receptor catabolic process; IDA:BHF-UCL.
 GO:0007179; P:transforming growth factor beta receptor signaling pathway; TAS:Reactome.
 GO:0030579; P:ubiquitin-dependent SMAD protein catabolic process; IDA:BHF-UCL. 
Interpro
 IPR000008; C2_Ca-dep.
 IPR008973; C2_Ca/lipid-bd_dom_CaLB.
 IPR018029; C2_membr_targeting.
 IPR024928; E3_ub_ligase_SMURF1.
 IPR000569; HECT.
 IPR001202; WW_dom. 
Pfam
 PF00168; C2
 PF00632; HECT
 PF00397; WW 
SMART
 SM00239; C2
 SM00119; HECTc
 SM00456; WW 
PROSITE
 PS50004; C2
 PS50237; HECT
 PS01159; WW_DOMAIN_1
 PS50020; WW_DOMAIN_2 
PRINTS