| Tag | Content |
|---|
| CPLM ID | CPLM-002831 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Colicin-E9 |
Protein Synonyms/Alias | |
Gene Name | col |
Gene Synonyms/Alias | cei |
Created Date | July 27, 2013 |
Organism | Escherichia coli |
NCBI Taxa ID | 562 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 517 | SKDPELSKNLNPSNK | acetylation | [1] | | 524 | KNLNPSNKSSVSKGY | acetylation | [1] | | 529 | SNKSSVSKGYSPFTP | acetylation | [1] | | 537 | GYSPFTPKNQQVGGR | acetylation | [1] | | 545 | NQQVGGRKVYELHHD | acetylation | [1] |
|
Reference | [1] Analysis of protein-protein interaction surfaces using a combination of efficient lysine acetylation and nanoLC-MALDI-MS/MS applied to the E9:Im9 bacteriotoxin--immunity protein complex. Scholten A, Visser NF, van den Heuvel RH, Heck AJ. J Am Soc Mass Spectrom. 2006 Jul;17(7):983-94. [ PMID: 16713291] |
Functional Description | This plasmid-coded bactericidal protein is an endonuclease active on both single- and double-stranded DNA but with undefined specificity. |
Sequence Annotation | METAL 550 550 Zinc (By similarity).
METAL 575 575 Zinc (By similarity).
METAL 579 579 Zinc (By similarity). |
Keyword | 3D-structure; Antibiotic; Antimicrobial; Bacteriocin; Endonuclease; Hydrolase; Metal-binding; Nuclease; Plasmid; Zinc. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 582 AA |
Protein Sequence | MSGGDGRGHN TGAHSTSGNI NGGPTGIGVS GGASDGSGWS SENNPWGGGS GSGIHWGGGS 60
GRGNGGGNGN SGGGSGTGGN LSAVAAPVAF GFPALSTPGA GGLAVSISAS ELSAAIAGII 120
AKLKKVNLKF TPFGVVLSSL IPSEIAKDDP NMMSKIVTSL PADDITESPV SSLPLDKATV 180
NVNVRVVDDV KDERQNISVV SGVPMSVPVV DAKPTERPGV FTASIPGAPV LNISVNDSTP 240
AVQTLSPGVT NNTDKDVRPA GFTQGGNTRD AVIRFPKDSG HNAVYVSVSD VLSPDQVKQR 300
QDEENRRQQE WDATHPVEAA ERNYERARAE LNQANEDVAR NQERQAKAVQ VYNSRKSELD 360
AANKTLADAI AEIKQFNRFA HDPMAGGHRM WQMAGLKAQR AQTDVNNKQA AFDAAAKEKS 420
DADAALSAAQ ERRKQKENKE KDAKDKLDKE SKRNKPGKAT GKGKPVGDKW LDDAGKDSGA 480
PIPDRIADKL RDKEFKSFDD FRKAVWEEVS KDPELSKNLN PSNKSSVSKG YSPFTPKNQQ 540
VGGRKVYELH HDKPISQGGE VYDMDNIRVT TPKRHIDIHR GK 582 |
Gene Ontology | GO:0005727; C:extrachromosomal circular DNA; IEA:InterPro. GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW. GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. GO:0019835; P:cytolysis; IEA:UniProtKB-KW. GO:0042742; P:defense response to bacterium; IEA:UniProtKB-KW. GO:0090305; P:nucleic acid phosphodiester bond hydrolysis; IEA:GOC. GO:0009405; P:pathogenesis; IEA:InterPro. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |