UniProt Accession

 NPM_MOUSE; Q61937 

Genbank Protein ID

 M33212; AK028253; BC054755 

Genbank Nucleotide ID

 AAA39801.1; BAC25844.1; AAH54755.1 

Protein Name

 Nucleophosmin 

Protein Synonyms/Alias

 NPM; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Numatrin 

Gene Name

 Npm1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
150	SAPGGGNKVPQKKVK	acetylation	[1]
154	GGNKVPQKKVKLDED	acetylation	[1]
211	QKSNQNGKDLKPSTP	acetylation	[1]
214	NQNGKDLKPSTPRSK	acetylation	[1]
227	SKGQESFKKQEKTPK	acetylation	[1]
231	ESFKKQEKTPKTPKG	acetylation	[1]
265	SLPKVEAKFINYVKN	acetylation	[1]

Reference

 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123] 

Functional Description

 Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules (By similarity). Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication (By similarity). Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation (By similarity). 

Sequence Annotation

 REGION 1 185 Required for interaction with SENP3 (By
 REGION 1 117 Necessary for interaction with APEX1 (By
 REGION 241 292 Required for nucleolar localization (By
 MOTIF 152 157 Nuclear localization signal (Potential).
 MOTIF 190 196 Nuclear localization signal (Potential).
 MOD_RES 1 1 N-acetylmethionine (By similarity).
 MOD_RES 4 4 Phosphoserine; by PLK1 and PLK2 (By
 MOD_RES 10 10 Phosphoserine (By similarity).
 MOD_RES 32 32 N6-acetyllysine (By similarity).
 MOD_RES 70 70 Phosphoserine.
 MOD_RES 75 75 Phosphothreonine (By similarity).
 MOD_RES 95 95 Phosphothreonine (By similarity).
 MOD_RES 125 125 Phosphoserine.
 MOD_RES 139 139 Phosphoserine (By similarity).
 MOD_RES 150 150 N6-acetyllysine (By similarity).
 MOD_RES 154 154 N6-acetyllysine (By similarity).
 MOD_RES 198 198 Phosphothreonine; by CDK1 and CDK2 (By
 MOD_RES 211 211 N6-acetyllysine (By similarity).
 MOD_RES 217 217 Phosphothreonine; by CDK1 (By
 MOD_RES 225 225 Phosphoserine (By similarity).
 MOD_RES 227 227 N6-acetyllysine (By similarity).
 MOD_RES 228 228 N6-acetyllysine; alternate (By
 MOD_RES 232 232 Phosphothreonine; by CDK1 (By
 MOD_RES 235 235 Phosphothreonine; by CDK1 (By
 MOD_RES 240 240 Phosphoserine (By similarity).
 MOD_RES 241 241 Phosphoserine (By similarity).
 MOD_RES 248 248 N6-acetyllysine (By similarity).
 MOD_RES 252 252 Phosphoserine.
 MOD_RES 255 255 N6-acetyllysine (By similarity).
 MOD_RES 258 258 Phosphoserine.
 MOD_RES 265 265 N6-acetyllysine (By similarity).
 MOD_RES 271 271 N6-acetyllysine (By similarity).
 MOD_RES 277 277 Phosphothreonine (By similarity).
 MOD_RES 290 290 N6-acetyllysine (By similarity).
 CROSSLNK 228 228 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 261 261 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Acetylation; ADP-ribosylation; Chaperone; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Disulfide bond; DNA-binding; Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome; RNA-binding; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 292 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; ISS:UniProtKB.
 GO:0005829; C:cytosol; IDA:MGI.
 GO:0001652; C:granular component; IDA:MGI.
 GO:0015934; C:large ribosomal subunit; IDA:MGI.
 GO:0016607; C:nuclear speck; IDA:MGI.
 GO:0015935; C:small ribosomal subunit; IDA:MGI.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0051059; F:NF-kappaB binding; ISS:UniProtKB.
 GO:0004860; F:protein kinase inhibitor activity; ISS:UniProtKB.
 GO:0043023; F:ribosomal large subunit binding; ISO:MGI.
 GO:0043024; F:ribosomal small subunit binding; ISO:MGI.
 GO:0019843; F:rRNA binding; IDA:MGI.
 GO:0051082; F:unfolded protein binding; ISS:UniProtKB.
 GO:0007569; P:cell aging; ISS:UniProtKB.
 GO:0016049; P:cell growth; IDA:MGI.
 GO:0006884; P:cell volume homeostasis; IDA:MGI.
 GO:0007098; P:centrosome cycle; ISS:UniProtKB.
 GO:0006281; P:DNA repair; ISS:UniProtKB.
 GO:0043066; P:negative regulation of apoptotic process; ISS:UniProtKB.
 GO:0008285; P:negative regulation of cell proliferation; ISS:UniProtKB.
 GO:0048025; P:negative regulation of mRNA splicing, via spliceosome; IDA:MGI.
 GO:0044387; P:negative regulation of protein kinase activity by regulation of protein phosphorylation; ISS:UniProtKB.
 GO:0008284; P:positive regulation of cell proliferation; IDA:MGI.
 GO:0010825; P:positive regulation of centrosome duplication; IGI:MGI.
 GO:0045860; P:positive regulation of protein kinase activity; IDA:MGI.
 GO:0045727; P:positive regulation of translation; ISS:UniProtKB.
 GO:0008104; P:protein localization; IMP:MGI.
 GO:0051259; P:protein oligomerization; ISO:MGI.
 GO:0046599; P:regulation of centriole replication; ISS:UniProtKB.
 GO:0043516; P:regulation of DNA damage response, signal transduction by p53 class mediator; IGI:MGI.
 GO:0060735; P:regulation of eIF2 alpha phosphorylation by dsRNA; ISS:UniProtKB.
 GO:0032071; P:regulation of endodeoxyribonuclease activity; ISS:UniProtKB.
 GO:0060699; P:regulation of endoribonuclease activity; ISS:UniProtKB.
 GO:0042273; P:ribosomal large subunit biogenesis; IMP:MGI.
 GO:0000055; P:ribosomal large subunit export from nucleus; IMP:MGI.
 GO:0042274; P:ribosomal small subunit biogenesis; IMP:MGI.
 GO:0000056; P:ribosomal small subunit export from nucleus; IDA:MGI.
 GO:0006407; P:rRNA export from nucleus; IDA:MGI. 

Interpro

 IPR004301; Nucleoplasmin.
 IPR024057; Nucleoplasmin_core_dom. 

Pfam

  

SMART

  

PROSITE

  

PRINTS