CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023023
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 DNA helicase MCM8 
Protein Synonyms/Alias
 Minichromosome maintenance 8 
Gene Name
 MCM8 
Gene Synonyms/Alias
 C20orf154 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
60PQFLLSTKTPQSMQSubiquitination[1]
101EKIQAFEKFFTRHIDubiquitination[1]
118DKDEIERKGSILVDFubiquitination[1]
208YEPLTQLKNVRANYYubiquitination[1]
232VVRVSNIKPLCTKMAubiquitination[1]
263GKYSLPTKCPVPVCRubiquitination[1]
460VGDPGLGKSQMLQAAubiquitination[1]
569PVGGHYNKAKTVSENubiquitination[1]
578KTVSENLKMGSALLSubiquitination[1]
644VLEVVSEKPLSERLKubiquitination[1]
651KPLSERLKVVPGETIubiquitination[1, 2]
668IPHQLLRKYIGYARQubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Component of the MCM8-MCM9 complex, a complex involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis. The MCM8- MCM9 complex probably acts as a hexameric helicase downstream of the Fanconi anemia proteins BRCA2 and RAD51 and is required to process aberrant forks into homologous recombination substrates and to orchestrate homologous recombination with resection, fork stabilization and fork restart. May also play a non-essential for DNA replication: may be involved in the activation of the prereplicative complex (pre-RC) during G(1) phase by recruiting CDC6 to the origin recognition complex (ORC). Binds chromatin throughout the cell cycle. 
Sequence Annotation
 DOMAIN 402 609 MCM.
 NP_BIND 454 461 ATP (Potential).  
Keyword
 Alternative splicing; ATP-binding; Cell cycle; Complete proteome; DNA damage; DNA repair; DNA replication; DNA-binding; Helicase; Hydrolase; Nucleotide-binding; Nucleus; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 840 AA 
Protein Sequence
MNGEYRGRGF GRGRFQSWKR GRGGGNFSGK WREREHRPDL SKTTGKRTSE QTPQFLLSTK 60
TPQSMQSTLD RFIPYKGWKL YFSEVYSDSS PLIEKIQAFE KFFTRHIDLY DKDEIERKGS 120
ILVDFKELTE GGEVTNLIPD IATELRDAPE KTLACMGLAI HQVLTKDLER HAAELQAQEG 180
LSNDGETMVN VPHIHARVYN YEPLTQLKNV RANYYGKYIA LRGTVVRVSN IKPLCTKMAF 240
LCAACGEIQS FPLPDGKYSL PTKCPVPVCR GRSFTALRSS PLTVTMDWQS IKIQELMSDD 300
QREAGRIPRT IECELVHDLV DSCVPGDTVT ITGIVKVSNA EEGSRNKNDK CMFLLYIEAN 360
SISNSKGQKT KSSEDGCKHG MLMEFSLKDL YAIQEIQAEE NLFKLIVNSL CPVIFGHELV 420
KAGLALALFG GSQKYADDKN RIPIRGDPHI LVVGDPGLGK SQMLQAACNV APRGVYVCGN 480
TTTTSGLTVT LSKDSSSGDF ALEAGALVLG DQGICGIDEF DKMGNQHQAL LEAMEQQSIS 540
LAKAGVVCSL PARTSIIAAA NPVGGHYNKA KTVSENLKMG SALLSRFDLV FILLDTPNEH 600
HDHLLSEHVI AIRAGKQRTI SSATVARMNS QDSNTSVLEV VSEKPLSERL KVVPGETIDP 660
IPHQLLRKYI GYARQYVYPR LSTEAARVLQ DFYLELRKQS QRLNSSPITT RQLESLIRLT 720
EARARLELRE EATKEDAEDI VEIMKYSMLG TYSDEFGNLD FERSQHGSGM SNRSTAKRFI 780
SALNNVAERT YNNIFQFHQL RQIAKELNIQ VADFENFIGS LNDQGYLLKK GPKVYQLQTM 840 
Gene Ontology
 GO:0097362; C:MCM8-MCM9 complex; IDA:UniProtKB.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0004386; F:helicase activity; IEA:UniProtKB-KW.
 GO:0006271; P:DNA strand elongation involved in DNA replication; TAS:Reactome.
 GO:0000724; P:double-strand break repair via homologous recombination; IEA:Compara.
 GO:0007292; P:female gamete generation; ISS:UniProtKB.
 GO:0000082; P:G1/S transition of mitotic cell cycle; TAS:Reactome.
 GO:0048232; P:male gamete generation; ISS:UniProtKB.
 GO:0006974; P:response to DNA damage stimulus; IDA:UniProtKB. 
Interpro
 IPR003593; AAA+_ATPase.
 IPR001208; MCM_DNA-dep_ATPase.
 IPR012340; NA-bd_OB-fold.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00493; MCM 
SMART
 SM00382; AAA
 SM00350; MCM 
PROSITE
 PS00847; MCM_1
 PS50051; MCM_2 
PRINTS
 PR01657; MCMFAMILY.