CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008750
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Sodium/myo-inositol cotransporter 
Protein Synonyms/Alias
 Na(+)/myo-inositol cotransporter; Sodium/myo-inositol transporter 1; SMIT1; Solute carrier family 5 member 3 
Gene Name
 SLC5A3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
115TMPEYLSKRFGGHRIubiquitination[1, 2, 3, 4]
249SPKKEALKMLRNPTDubiquitination[2]
290VQRVLAAKNIAHAKGubiquitination[2, 4]
549RTTTFWSKKNLVVKEubiquitination[2]
555SKKNLVVKENCSPKEubiquitination[5]
590NHIIPNGKSEDSIKGubiquitination[3]
596GKSEDSIKGLQPEDVubiquitination[2, 4, 5, 6]
642QAALMGEKERKKETDubiquitination[1, 2, 3]
665IDWFCGFKSKSLSKRubiquitination[2, 5]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 
Functional Description
 Prevents intracellular accumulation of high concentrations of myo-inositol (an osmolyte) that result in impairment of cellular function. 
Sequence Annotation
 CARBOHYD 232 232 N-linked (GlcNAc...) (Potential).  
Keyword
 Complete proteome; Glycoprotein; Ion transport; Membrane; Polymorphism; Reference proteome; Sodium; Sodium transport; Symport; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 718 AA 
Protein Sequence
MRAVLDTADI AIVALYFILV MCIGFFAMWK SNRSTVSGYF LAGRSMTWVT IGASLFVSNI 60
GSEHFIGLAG SGAASGFAVG AWEFNALLLL QLLGWVFIPI YIRSGVYTMP EYLSKRFGGH 120
RIQVYFAALS LILYIFTKLS VDLYSGALFI QESLGWNLYV SVILLIGMTA LLTVTGGLVA 180
VIYTDTLQAL LMIIGALTLM IISIMEIGGF EEVKRRYMLA SPDVTSILLT YNLSNTNSCN 240
VSPKKEALKM LRNPTDEDVP WPGFILGQTP ASVWYWCADQ VIVQRVLAAK NIAHAKGSTL 300
MAGFLKLLPM FIIVVPGMIS RILFTDDIAC INPEHCMLVC GSRAGCSNIA YPRLVMKLVP 360
VGLRGLMMAV MIAALMSDLD SIFNSASTIF TLDVYKLIRK SASSRELMIV GRIFVAFMVV 420
ISIAWVPIIV EMQGGQMYLY IQEVADYLTP PVAALFLLAI FWKRCNEQGA FYGGMAGFVL 480
GAVRLILAFA YRAPECDQPD NRPGFIKDIH YMYVATGLFW VTGLITVIVS LLTPPPTKEQ 540
IRTTTFWSKK NLVVKENCSP KEEPYQMQEK SILRCSENNE TINHIIPNGK SEDSIKGLQP 600
EDVNLLVTCR EEGNPVASLG HSEAETPVDA YSNGQAALMG EKERKKETDD GGRYWKFIDW 660
FCGFKSKSLS KRSLRDLMEE EAVCLQMLEE TRQVKVILNI GLFAVCSLGI FMFVYFSL 718 
Gene Ontology
 GO:0005887; C:integral to plasma membrane; TAS:ProtInc.
 GO:0005367; F:myo-inositol:sodium symporter activity; TAS:ProtInc.
 GO:0006020; P:inositol metabolic process; IEA:Compara.
 GO:0007422; P:peripheral nervous system development; IEA:Compara.
 GO:0043576; P:regulation of respiratory gaseous exchange; IEA:Compara. 
Interpro
 IPR001734; Na/solute_symporter.
 IPR018212; Na/solute_symporter_CS.
 IPR019900; Na/solute_symporter_subgr. 
Pfam
 PF00474; SSF 
SMART
  
PROSITE
 PS00456; NA_SOLUT_SYMP_1
 PS00457; NA_SOLUT_SYMP_2
 PS50283; NA_SOLUT_SYMP_3 
PRINTS